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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   ULK1 Antibody   

ULK1 Antibody

  • WB - ULK1 Antibody ASC11653
    Western blot analysis of ULK1 in rat brain tissue lysate with ULK1 antibody at 1 µg/mL in (A) the absence and (B) the presence of blocking peptide.
  • IHC - ULK1 Antibody ASC11653
    Immunohistochemistry of ULK1 in rat brain tissue with ULK1 antibody at 2.5 µg/ml.
  • IF - ULK1 Antibody ASC11653
    Immunofluorescence of ULK1 in rat brain tissue with ULK1 antibody at 20 µg/ml.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession O75385
Other Accession NP_003556, 4507831
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Isotype IgG
Calculated MW Predicted: 116 kDa

Observed: 105 kDa
Application Notes ULK1 Antibody can be used for detection of ULK1 by Western blot at 1 µg/mL.
Additional Information
Gene ID 8408
Target/Specificity ULK1; Two alternatively spliced transcript variants encoding different isoforms have been identified. ULK1 antibody is predicted to not cross-react with ULK2.
Reconstitution & Storage ULK1 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year.
PrecautionsULK1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name ULK1
Synonyms KIAA0722
Function Serine/threonine-protein kinase involved in autophagy in response to starvation. Acts upstream of phosphatidylinositol 3- kinase PIK3C3 to regulate the formation of autophagophores, the precursors of autophagosomes. Part of regulatory feedback loops in autophagy: acts both as a downstream effector and negative regulator of mammalian target of rapamycin complex 1 (mTORC1) via interaction with RPTOR. Activated via phosphorylation by AMPK and also acts as a regulator of AMPK by mediating phosphorylation of AMPK subunits PRKAA1, PRKAB2 and PRKAG1, leading to negatively regulate AMPK activity. May phosphorylate ATG13/KIAA0652 and RPTOR; however such data need additional evidences. Plays a role early in neuronal differentiation and is required for granule cell axon formation.
Cellular Location Cytoplasm, cytosol. Preautophagosomal structure. Note=Under starvation conditions, is localized to puncate structures primarily representing the isolation membrane that sequesters a portion of the cytoplasm resulting in the formation of an autophagosome
Tissue Location Ubiquitously expressed. Detected in the following adult tissues: skeletal muscle, heart, pancreas, brain, placenta, liver, kidney, and lung
Research Areas
Citations (0)

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ULK1 Antibody: ULK1, also known as ATG1, is a key serine/threonine protein kinase probably acting at the most upstream step of autophagosome formation. Knockout of ULK1 results in a severe defect in the autophagy pathway. ULK1 is highly conserved among eukaryotes, and are the Unc-51-like kinases, ULK1 and ULK2 in mammals. ULK1 is ubiquitously expressed and involved in autophagy in response to starvation. It is the target of the TOR kinase signaling pathway that regulates autophagy through the control of phosphorylation status of ATG13. ULK1 also plays a role early in neuronal differentiation.


Suzuki K, Kubota Y, Sekito T, et al. Hierarchy of Atg proteins in pre-autophagosomal structure organization. Genes to Cells 2007; 12:209–18.
Lee EJ and Tournier C. The requirement of uncoordinated 51-like kinase 1 (ULK1) and ULK2 in the regulation of autophagy. Autophagy 2011; 7:689-95.
Yan J, Kuroyanagi H, Tomemori T, et al. Mouse ULK2, a novel member of the UNC-51-like protein kinases: unique features of functional domains. Oncogene 1999; 18:5850–9.
Bach M, Larance M, James DE, et al. The serine/threonine kinase ULK1 is a target of multiple phosphorylation events. Biochem. J. 2011; 440:283-91.

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$ 310.00
Cat# ASC11653
(40 western blots)
Availability: 5-7days
Bulk Size
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