|Application ||WB, IHC-P, IF, E|
|Other Accession||NP_055768, 190014623|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||Predicted: 105 kDa |
Observed: 103 kDa
|Application Notes||DIS3 antibody can be used for detection of DIS3 by Western blot at 1 - 2 µg/mL.|
|Target/Specificity||DIS3; Multiple isoforms of DIS3 are known to exist.|
|Reconstitution & Storage||DIS3 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year.|
|Precautions||DIS3 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Putative catalytic component of the RNA exosome complex which has 3'->5' exoribonuclease activity and participates in a multitude of cellular RNA processing and degradation events. In the nucleus, the RNA exosome complex is involved in proper maturation of stable RNA species such as rRNA, snRNA and snoRNA, in the elimination of RNA processing by-products and non-coding 'pervasive' transcripts, such as antisense RNA species and promoter-upstream transcripts (PROMPTs), and of mRNAs with processing defects, thereby limiting or excluding their export to the cytoplasm. The RNA exosome may be involved in Ig class switch recombination (CSR) and/or Ig variable region somatic hypermutation (SHM) by targeting AICDA deamination activity to transcribed dsDNA substrates. In the cytoplasm, the RNA exosome complex is involved in general mRNA turnover and specifically degrades inherently unstable mRNAs containing AU-rich elements (AREs) within their 3' untranslated regions, and in RNA surveillance pathways, preventing translation of aberrant mRNAs. It seems to be involved in degradation of histone mRNA. DIS3 has both 3'-5' exonuclease and endonuclease activities.|
|Cellular Location||Cytoplasm. Nucleus, nucleolus. Nucleus, nucleoplasm. Nucleus. Note=Predominantly located in the nucleus (PubMed:20531386). According to PubMed:12429849, found in the nucleolus (PubMed:12429849). According to PubMed:20531386, excluded from nucleolus supporting the existence of a nucleolar RNA exosome complex devoid of DIS3 (PubMed:20531386)|
|Tissue Location||Widely expressed.|
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Provided below are standard protocols that you may find useful for product applications.
DIS3 Antibody: The exosome is involved in a multitude of cellular RNA processing and degradation events. DIS3, also known as exosome complex exonuclease RRP44, is a ribonuclease that acts directly in the processing, turnover, and surveillance of a large number of distinct RNA species. DIS3 localizes to both the cytoplasm and the nucleus and contains one PINc domain. It is widely expressed with highest expression in testis and is required for processing of 7S pre-RNA into a mature nuclear complex and, ultimately, for proper mitotic progression. Abnormal expression levels of DIS3 may be associated with colon cancer, suggesting a role for DIS3 in tumorigenesis.
Chen CY, Gherzi R, Ong SE, et al. AU binding proteins recruit the exosome to degrade ARE-containing mRNAs. Cell 2001; 107:451-64.
Brouwer R, Allmang C, Raijmakers R, et al. Three novel components of the human exosome. J. Biol. Chem. 2001; 276:6177-84.
Schneider C, Anderson JT, Tollervey D, et al. The exosome subunit Rrp44 plays a direct role in RNA substrate recognition. Mol. Cell 2007; 27:324-31.
Murakami H, Goto DB, Toda T, et al. Ribonuclease activity of DIS3 is required for mitotic progression and provides a possible link between heterochromatin and kinetochore function. PLoS ONE 2007; 2:317.
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