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GABARAP Antibody

  • WB - GABARAP Antibody ASC11660
    Western blot analysis of GABARAP in K562 cell lysate with GABARAP antibody at (A) 1 and (B) 2 µg/mL.
  • IF - GABARAP Antibody ASC11660
    Immunofluorescence of GABARAP in human brain tissue with GABARAP antibody at 20 µg/ml.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession O95166
Other Accession NP_009209, 6005764
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Isotype IgG
Calculated MW Predicted: 13

Observed: 16 KDa
Application Notes GABARAP antibody can be used for detection of GABARAP by Western blot at 1 - 2 µg/mL.
Additional Information
Gene ID 11337
Target/Specificity GABARAP; Multiple isoforms of GABARAP exist due to alternative splicing events.
Reconstitution & Storage GABARAP antibody can be stored at 4℃ for three months and -20℃, stable for up to one year.
PrecautionsGABARAP Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Synonyms FLC3B
Function Ubiquitin-like modifier that plays a role in intracellular transport of GABA(A) receptors and its interaction with the cytoskeleton. Involved in apoptosis. Involved in autophagy. Whereas LC3s are involved in elongation of the phagophore membrane, the GABARAP/GATE-16 subfamily is essential for a later stage in autophagosome maturation.
Cellular Location Endomembrane system. Cytoplasm, cytoskeleton. Golgi apparatus membrane. Cytoplasmic vesicle, autophagosome Note=Largely associated with intracellular membrane structures including the Golgi apparatus and postsynaptic cisternae Colocalizes with microtubules (By similarity). Localizes also to discrete punctae along the ciliary axoneme (By similarity)
Tissue Location Heart, brain, placenta, liver, skeletal muscle, kidney and pancreas.
Research Areas
Citations (0)

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GABARAP Antibody: Gamma-aminobutyric acid (GABA) is the main inhibitory transmitter by increasing a Cl-conductance that inhibits neuronal firing in the central nervous system. It has been shown to activate both ionotropic (GABAA) and metabotropic (GABAB) receptors as well as a third class of receptors called GABAC. GABARAP (GABAA receptor-associated protein) links GABAA receptors to the cytoskeleton and may play a role in intracellular transport of GABAA receptors and its interaction with the cytoskeleton. GABARAP belongs to the MAP1 or ATG8 like family and recent studies show that MAPK15/ERK8 is acting through interaction with ATG8 family proteins to regulate autophagy.


Cherubini E, Gaiarsa JL, and Ben-Ari Y. GABA: an excitatory transmitter in early postnatal life. Trends Neurosci.1991; 14:515-19.
Dirkx R Jr, Thomas A, Li L, et al. Targeting of the 67 kDa isoform of glutamic acid decarboxylase to intracellular organelles is mediated by its interaction with the NH2-terminal region of the 65 kDa isoform of glutamic acid decarboxylase. J. Biol. Chem. 1995; 270:2241-6.
Wang H, Bedford FK, Brandon NJ, et al. GABA(A)-receptor-associated protein links GABAA receptors and the cytoskeleton. Nature 1999; 397:69-72.
Colecchia D, Strambi A, Sanzone S, et al. MAPK15/ERK8 stimulates autophagy by interacting with LC3 and GABARAP proteins. Autophagy 2012 Sep 4;8(12).

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$ 310.00
Cat# ASC11660
(40 western blots)
Availability: 5-7days
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