|Application ||WB, IHC-P, IF, E|
|Other Accession||NP_004385, 4758120|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||Predicted: 11 kDa |
Observed: 11 kDa
|Application Notes||DAP antibody can be used for detection of DAP by Western blot at 1 - 2 µg/mL.|
|Target/Specificity||DAP; DAP antibody is human, mouse and rat reactive.|
|Reconstitution & Storage||DAP antibody can be stored at 4℃ for three months and -20℃, stable for up to one year.|
|Precautions||DAP Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Negative regulator of autophagy. Involved in mediating interferon-gamma-induced cell death.|
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Provided below are standard protocols that you may find useful for product applications.
DAP Antibody: The Death-associated protein (DAP) is a basic proline-rich 15kDa protein that as a positive mediator of programmed cell death that is induced by interferon-gamma (1). DAP is also a direct substrate of mammalian target of rapamycin (mTOR), a serine/threonine kinase that regulates cell growth and cell cycle, and a negative regulator of autophagy. Under rich nutrient conditions, mTOR phosphorylates DAP at Ser3 and Ser51; under starvation conditions, these residues are dephosphorylated and DAP is converted into an active suppressor of autophagy (2).
Deiss LP, Feinstein E, Berissi H, et al. Identification of a novel serine/threonine kinase and a novel 15-kD protein as potential mediators of the gamma interferon-induced cell death. Genes Dev. 1995; 9:15-30.
Koren I, Reem E, and Kimchi A. DAP1, a novel substrate of mTOR, negatively regulates autophagy. Curr. Biol. 2010; 20:1093-8.
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