|Application ||WB, IHC-P, IF, E|
|Other Accession||NP_001238759, 352962176|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||Predicted: 36 kDa |
Observed: 37 kDa
|Application Notes||SPP1 antibody can be used for detection of SPP1 by Western blot at 1 - 2 µg/mL.|
|Target/Specificity||SPP1; SPP1 antibody is human, mouse and rat reactive. Multiple isoforms of SPP1 are known to exist.|
|Reconstitution & Storage||SPP1 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year.|
|Precautions||SPP1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Binds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction.|
|Tissue Location||Bone. Found in plasma.|
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Provided below are standard protocols that you may find useful for product applications.
SPP1 Antibody: The secreted protein 1 (SPP1), also known as osteopontin, is a major noncollagenous protein of bone, but is also found in the extracellular matrix of other mineralized tissues and in bodily fluids. In bone, SPP1 is produced by osteoblasts, osteocytes, macrophages, and osteoclasts (1,2). SPP1 binds to cells through integrin and non-integrin receptors, as well as the adhesion receptor CD44 in an RGD-independent manner, enabling SPP1 to induce a number of functional effects including macrophage chemotaxis, cytoprotection, and regulation of T helper type 1 cells (2). SPP1 can regulate biomineralization by inhibiting the formation of hydroxyapatite (3) and the growth of calcium oxalate crystals (4).
Heinegard D, Andersson G, and Reinholt FP. Roles of osteopontin in bone remodeling. Ann. N.Y. Acad. Sci.1995; 760:213-22.
Wang KX and Denhardt DT. Osteopontin: rule in immune regulation and stress responses. Cyto. Growth Factor Rev. 2008; 19:333-45.
Boskey AL, Maresca M, Ullrich W, et al. Osteopontin-hydroxyapatite interactions in vitro: inhibition of hydroxyapatite formation and growth in a gelatin-gel. Bone Miner. 1993; 22:147-159.
Shiraga H, Min W, VanDusen WJ, et al. Inhibition of calcium oxalate crystal growth in vitro by uropontin: another member of the aspartic acid-rich protein superfamily. Proc. Natl. Acad. Sci. USA 1992; 89:426-30.
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