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WFDC2 Antibody

     
  • WB - WFDC2 Antibody  ASC11692
    Western blot analysis of WFDC2 in A549 cell lysate with WFDC2 antibody at 1 µg/ml in (A) the absence and (B) the presence of blocking peptide.
  • IHC - WFDC2 Antibody  ASC11692
    Immunohistochemistry of WFDC2 in human lung tissue with WFDC2 antibody at 2.5 µg/mL.
  • IF - WFDC2 Antibody  ASC11692
    Immunofluorescence of WFDC2 in human lung tissue with WFDC2 antibody at 20 µg/mL.
  • SPECIFICATION
  • CITATIONS
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, IF, E
Primary Accession Q14508
Other Accession NP_006094, 56699495
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype IgG
Calculated MW Predicted: 14 kDa

Observed: 20kDa
Application Notes WFDC2 antibody can be used for detection of WFDC2 by Western blot at 1 - 2 µg/ml.
Additional Information
Gene ID 10406
Target/Specificity WFDC2; WFDC2 antibody is human specific. Multiple isoforms of WFDC2 are known to exist.
Reconstitution & Storage WFDC2 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year.
PrecautionsWFDC2 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name WFDC2
Synonyms HE4, WAP5
Function Broad range protease inhibitor.
Cellular Location Secreted
Tissue Location Expressed in a number of normal tissues, including male reproductive system, regions of the respiratory tract and nasopharynx. Highly expressed in a number of tumors cells lines, such ovarian, colon, breast, lung and renal cells lines. Initially described as being exclusively transcribed in the epididymis. EMBL; X63187; CAA44869.1; -; mRNA EMBL; AF330259; AAL37485.1; -; mRNA EMBL; AF330260; AAL37486.1; -; mRNA EMBL; AF330261; AAL37487.1; -; mRNA EMBL; AF330262; AAL37488.1; -; mRNA EMBL; AY212888; AAO52683.1; -; mRNA EMBL; CR456977; CAG33258.1; -; mRNA EMBL; AL031663; -; NOT_ANNOTATED_CDS; Genomic_DNA EMBL; CH471077; EAW75836.1; -; Genomic_DNA EMBL; CH471077; EAW75837.1; -; Genomic_DNA EMBL; CH471077; EAW75839.1; -; Genomic_DNA EMBL; BC046106; AAH46106.1; -; mRNA CCDS; CCDS35501.1; -. [Q14508-1] PIR; S25454; S25454 RefSeq; NP_006094.3; NM_006103.3. [Q14508-1] UniGene; Hs.2719; - ProteinModelPortal; Q14508; - SMR; Q14508; - BioGrid; 115677; 14 IntAct; Q14508; 15 STRING; 9606.ENSP00000361761; - MEROPS; I17.004; - iPTMnet; Q14508; - BioMuta; WFDC2; - DMDM; 20141958; - EPD; Q14508; - MaxQB; Q14508; - PaxDb; Q14508; - PeptideAtlas; Q14508; - PRIDE; Q14508; - ProteomicsDB; 60010; - ProteomicsDB; 60011; -. [Q14508-2] ProteomicsDB; 60012; -. [Q14508-3] ProteomicsDB; 60013; -. [Q14508-4] ProteomicsDB; 60014; -. [Q14508-5] DNASU; 10406; - Ensembl; ENST00000217425; ENSP00000217425; ENSG00000101443. [Q14508-5] Ensembl; ENST00000339946; ENSP00000340215; ENSG00000101443. [Q14508-3] Ensembl; ENST00000342873; ENSP00000342890; ENSG00000101443. [Q14508-2] Ensembl; ENST00000372676; ENSP00000361761; ENSG00000101443. [Q14508-1] GeneID; 10406; - KEGG; hsa:10406; - UCSC; uc002xoo.4; human. [Q14508-1] CTD; 10406; - DisGeNET; 10406; - EuPathDB; HostDB:ENSG00000101443.17; - GeneCards; WFDC2; - HGNC; HGNC:15939; WFDC2 HPA; HPA042302; - MIM; 617548; gene neXtProt; NX_Q14508; - OpenTargets; ENSG00000101443; - PharmGKB; PA38059; - eggNOG; ENOG410J36N; Eukaryota eggNOG; ENOG41125F3; LUCA GeneTree; ENSGT00730000111410; - HOVERGEN; HBG018073; - InParanoid; Q14508; - OMA; DLNCTQE; - OrthoDB; EOG091G0UW4; - PhylomeDB; Q14508; - ChiTaRS; WFDC2; human GeneWiki; WFDC2; - GenomeRNAi; 10406; - PRO; PR:Q14508; - Proteomes; UP000005640; Chromosome 20 Bgee; ENSG00000101443; - CleanEx; HS_WFDC2; - ExpressionAtlas; Q14508; baseline and differential Genevisible; Q14508; HS GO; GO:0070062; C:extracellular exosome; HDA:UniProtKB GO; GO:0005615; C:extracellular space; TAS:ProtInc GO; GO:0019828; F:aspartic-type endopeptidase inhibitor activity; IDA:CACAO GO; GO:0004869; F:cysteine-type endopeptidase inhibitor activity; IEA:UniProtKB-KW GO; GO:0004866; F:endopeptidase inhibitor activity; TAS:ProtInc GO; GO:0004867; F:serine-type endopeptidase inhibitor activity; IDA:CACAO GO; GO:0006508; P:proteolysis; TAS:ProtInc GO; GO:0007283; P:spermatogenesis; TAS:ProtInc Gene3D; 4.10.75.10; -; 2 InterPro; IPR036645; Elafin-like_sf InterPro; IPR008197; WAP_dom Pfam; PF00095; WAP; 2 PRINTS; PR00003; 4DISULPHCORE SMART; SM00217; WAP; 2 SUPFAM; SSF57256; SSF57256; 2 PROSITE; PS51390; WAP; 2 1: Evidence at protein level; Alternative splicing; Aspartic protease inhibitor; Complete proteome; Disulfide bond; Glycoprotein; Protease inhibitor; Reference proteome; Repeat; Secreted; Serine protease inhibitor; Signal; Thiol protease inhibitor SIGNAL 1 30 CHAIN 31 124 WAP four-disulfide core domain protein 2 /FTId=PRO_0000041370 DOMAIN 31 73 WAP 1. {ECO:0000255|PROSITE- ProRule:PRU00722} DOMAIN 74 123 WAP 2. {ECO:0000255|PROSITE- ProRule:PRU00722} CARBOHYD 44 44 N-linked (GlcNAc...) asparagine DISULFID 36 62 {ECO:0000255|PROSITE-ProRule:PRU00722} DISULFID 45 66 {ECO:0000255|PROSITE-ProRule:PRU00722} DISULFID 49 61 {ECO:0000255|PROSITE-ProRule:PRU00722} DISULFID 55 70 {ECO:0000255|PROSITE-ProRule:PRU00722} DISULFID 80 110 {ECO:0000255|PROSITE-ProRule:PRU00722} DISULFID 93 114 {ECO:0000255|PROSITE-ProRule:PRU00722} DISULFID 97 109 {ECO:0000255|PROSITE-ProRule:PRU00722} DISULFID 103 119 {ECO:0000255|PROSITE-ProRule:PRU00722} VAR_SEQ 2 23 PACRLGPLAAALLLSLLLFGFT -> LQVQVNLPVSPLPTY PYSFFYP (in isoform 2) /FTId=VSP_007666 VAR_SEQ 24 74 Missing (in isoform 2) /FTId=VSP_007667 VAR_SEQ 27 74 Missing (in isoform 3) /FTId=VSP_007668 VAR_SEQ 71 79 SLPNDKEGS -> LLCPNGQLAE (in isoform 4) /FTId=VSP_007669 VAR_SEQ 75 102 DKEGSCPQVNINFPQLGLCRDQCQVDSQ -> ALFHWHLKT RRLWEISGPRPRRPTWDSS (in isoform 5) /FTId=VSP_007670 VAR_SEQ 80 124 Missing (in isoform 4) /FTId=VSP_007671 VAR_SEQ 103 124 Missing (in isoform 5) /FTId=VSP_007672 CONFLICT 71 72 SL -> LLC (in Ref. 1; CAA44869 and 2; AAL37485). CONFLICT 101 101 S -> T (in Ref. 1; CAA44869) SEQUENCE 124 AA; 12993 MW; 9536B00B385259AD CRC64; MPACRLGPLA AALLLSLLLF GFTLVSGTGA EKTGVCPELQ ADQNCTQECV SDSECADNLK CCSAGCATFC SLPNDKEGSC PQVNINFPQL GLCRDQCQVD SQCPGQMKCC RNGCGKVSCV TPNF
Citations (0)

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Background

The WAP four-disulfide core domain protein 2 (WFDC2), also known as epididymal protein 4, is a member of the WFDC domain family, a family of proteins that is characterized by the presence of Whey Acidic Protein (WAP) domain, and is highly expressed in the lung and salivary gland (1,2). Members of this family include SLPI and elafin, antiproteinases involved in the innate immune system (2). WFDC2 has been proposed to play a critical role in tumor formation and growth in ovarian cancer cells through the regulation of growth- and apoptosis-associated genes and may thus be a potential therapeutic target for epithelial ovarian cancer (3).

References

Drapkin R, von Horsten HH, Lin Y, et al. Human epididymis protein 4 (HE4) is a secreted glycoprotein that is overexpressed by serious and endometrioid ovarian carcinomas. Cancer Res. 2005; 65:2162-9.
Bingle L, Cross SS, High AS, et al. WFDC2 (HE4): A potential role in the innate immunity of the oral cavity and respiratory tract and the development of adenocarcinomas of the lung. Resp. Res. 2006; 7:61.
Chen Y, Mu X, Wang S, et al. WAP four-disulfide core domain protein 2 mediates the proliferation of human ovarian cancer cells through the regulation of growth- and apoptosis-associated genes. Oncol. Rep. 2013; 29:288-96.

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Cat# ASC11692
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