|Application ||WB, E|
|Other Accession||NP_000873, 24430219|
|Calculated MW||Predicted: 22 kDa |
Observed: 22 kDa
|Application Notes||IL-12A antibody can be used for detection of IL-12A by Western blot at 0.5 - 1 µg/ml.|
|Target/Specificity||IL 12A; IL-12A antibody is human specific. IL-12A antibody will not cross-react with IL-12B.|
|Reconstitution & Storage||IL-12A antibody can be stored at 4℃ for three months and -20℃, stable for up to one year.|
|Precautions||IL-12A Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Cytokine that can act as a growth factor for activated T and NK cells, enhance the lytic activity of NK/lymphokine- activated Killer cells, and stimulate the production of IFN-gamma by resting PBMC.|
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Provided below are standard protocols that you may find useful for product applications.
IL-12A is subunit of the cytokine IL-12 which acts on T- and natural killer cells, and has a broad array of biological activities. IL-12 is a disulfide-linked heterodimer composed of the 35-kD subunit and a 40-kD subunit that is a member of the cytokine receptor family (1). IL-12 is required for the T-cell-independent induction of interferon (IFN)-gamma, and is important for the differentiation of both Th1 and Th2 cells (1,2). The responses of lymphocytes to IL-12 are mediated by the activator of transcription protein STAT4 (2). Nitric oxide synthase 2A (NOS2A/NOS2) is found to be required for the signaling process of this cytokine in innate immunity (3).
Gee K, Guzzo C, Che Mat NF, et al. The IL-12 family of cytokines in infection, inflammation and autoimmune disorders. Inflamm. Allergy Drug Targets 2009; 8:40-52.
Sinigaglia F, D’Ambrosio D, Panina-Bordignon P, et al. Regulation of the IL-12/IL-12R axis: a critical step in T-helper cell differentiation and effector function. Immunol. Rev. 1999; 170:65-72.
Bogdan C, Rollinghoff M, and Diefenbach A. The role of nitric oxide in innate immunity. Immunol. Rev. 2000; 173:17-26.
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