|Application ||WB, E|
|Other Accession||NP_694587, 116256451|
|Calculated MW||Predicted: 65 kDa |
Observed: 68 kDa
|Application Notes||KDM1B antibody can be used for detection of KDM1B by Western blot at 1 - 2 µg/ml.|
|Target/Specificity||KDM1B; KDM1B antibody is human, mouse and rat reactive. At least two isoforms of KDM1B are known to exist; this KDM1B antibody will detect both isoforms.|
|Reconstitution & Storage||KDM1B antibody can be stored at 4℃ for three months and -20℃, stable for up to one year.|
|Precautions||KDM1B Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Synonyms||AOF1, C6orf193, LSD2|
|Function||Histone demethylase that demethylates 'Lys-4' of histone H3, a specific tag for epigenetic transcriptional activation, thereby acting as a corepressor. Required for de novo DNA methylation of a subset of imprinted genes during oogenesis. Acts by oxidizing the substrate by FAD to generate the corresponding imine that is subsequently hydrolyzed. Demethylates both mono- and di-methylated 'Lys-4' of histone H3. Has no effect on tri- methylated 'Lys-4', mono-, di- or tri-methylated 'Lys-9', mono-, di- or tri-methylated 'Lys-27', mono-, di- or tri-methylated 'Lys- 36' of histone H3, or on mono-, di- or tri-methylated 'Lys-20' of histone H4 (By similarity).|
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Provided below are standard protocols that you may find useful for product applications.
Flavin-dependent histone demethylases regulate histone lysine methylation, an epigenetic mark that regulates gene expression and chromatin function (reviewed in 1). KDM1B is a recently identified histone H3K4 demethylase that is required to establish maternal genomic imprints; targeted disruption of the KDM1B gene in mice led to lethality in their embryos, suggesting demethylation of H3K4 is critical for establishing the DNA methylation imprints (2). KDM1B has also been shown to play a role in the transcription elongation in several genes (3).
Culhane JC and Cole PA. LSD1 and the chemistry of histone demethylation. Curr. Opin. Chem. Biol. 2007; 11:561-8.
Ciccone DN, Su H, Hevi S, et al. KDM1B is a histone H3K4 demethylase required to establish maternal genomic imprints. Nature 2009; 461:415-8.
Fang R, Barbera A, Xu Y, et al. Human LSD2/KDM1b/AOF1 regulates genes transcription by modulating intragenic H3K4me2 methylation. Mol. Cell 2010; 39:222-233.
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