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AMIGO1 Antibody

  • WB - AMIGO1 Antibody ASC11725
    Western blot analysis of AMIGO1 in HeLa cell lysate with AMIGO1 antibody at (A) 1 and (B) 2 µg/ml.
  • IHC - AMIGO1 Antibody ASC11725
    Immunohistochemistry of AMIGO1 in human brain tissue with AMIGO1 antibody at 5 µg/mL.
  • IF - AMIGO1 Antibody ASC11725
    Immunofluorescence of AMIGO1 in human brain tissue with AMIGO1 antibody at 20 µg/mL.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q86WK6
Other Accession NP_065754, 153791466
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Isotype IgG
Calculated MW Predicted: 54 kDa

Observed: 55 kDa
Application Notes AMIGO1 antibody can be used for detection of AMIGO1 by Western blot at 1 - 2 µg/ml. Antibody can also be used for Immunohistochemistry starting at 5 µg/mL. For immunofluorescence start at 20 µg/mL.
Additional Information
Gene ID 57463
Target/Specificity AMIGO1; AMIGO1 antibody is human, mouse, and rat reactive. AMIGO1 antibody is predicted to not cross-react with AMIGO2 or AMIGO3.
Reconstitution & Storage AMIGO1 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year.
PrecautionsAMIGO1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name AMIGO1 (HGNC:20824)
Function Promotes growth and fasciculation of neurites from cultured hippocampal neurons. May be involved in fasciculation as well as myelination of developing neural axons. May have a role in regeneration as well as neural plasticity in the adult nervous system. May mediate homophilic as well as heterophilic cell-cell interaction and contribute to signal transduction through its intracellular domain (By similarity).
Cellular Location Cell membrane; Single-pass type I membrane protein. Cell projection, axon. Note=Associated with axons of neuronal cells
Research Areas
Citations (0)

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The amphoterin-induced gene and ORF (AMIGO1) protein is a brain-enriched, glycosylated transmembrane immunoglobulin (Ig) superfamily protein with six extracellular leucine-rich repeats (LRRs) and one Ig-like domain. It and the related proteins AMIGO2 and AMIGO3 are thought to be cell adhesion molecules expressed on fiber tracts of neuronal tissues and participate in their formation (1). AMIGO1 has also been suggested to play important roles in dendritic outgrowth during development and could modulate the survival of developing and adult neurons (2).


Kuja-Panula J, Kiiltomaki M, Yamashiro T, et al. AMIGO, a transmembrane protein implicated in axon tract development, defines a novel protein family with leucine-rich repeats. J. Cell Biol. 2003; 160:963-73.
Chen Y, Hor HH, and Tang BL. AMIGO is expressed in multiple brain cell types and may regulate dendritic growth and neuronal survival. J. Cell Physiol. 2012; 227:2217-29.

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$ 310.00
Cat# ASC11725
(40 western blots)
Availability: 5-7days
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