|Application ||WB, IHC-P, IF, E|
|Other Accession||NP_073729, 12383056|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||Predicted: 14 kDa |
Observed: 14 kDa
|Application Notes||MAP1LC3B antibody can be used for detection of MAP1LC3B by Western blot at 1 - 2 µg/ml. Antibody can also be used for Immunohistochemistry starting at 5 µg/mL. For immunofluorescence start at 20 µg/mL.|
|Target/Specificity||MAP1LC3B; MAP1LC3B antibody is human, mouse and rat reactive. Multiple isoforms MAP1LC3B are known to exist. MAP1LC3B antibody is predicted to not cross-react with MAP1LC3A or MAP1LC3C|
|Reconstitution & Storage||MAP1LC3B antibody can be stored at 4℃ for three months and -20℃, stable for up to one year.|
|Precautions||MAP1LC3B Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Ubiquitin-like modifier involved in formation of autophagosomal vacuoles (autophagosomes). Plays a role in mitophagy which contributes to regulate mitochondrial quantity and quality by eliminating the mitochondria to a basal level to fulfill cellular energy requirements and preventing excess ROS production. Whereas LC3s are involved in elongation of the phagophore membrane, the GABARAP/GATE-16 subfamily is essential for a later stage in autophagosome maturation. Promotes primary ciliogenesis by removing OFD1 from centriolar satellites via the autophagic pathway.|
|Cellular Location||Cytoplasm, cytoskeleton. Endomembrane system; Lipid-anchor. Cytoplasmic vesicle, autophagosome membrane; Lipid-anchor. Note=LC3-II binds to the autophagic membranes Localizes also to discrete punctae along the ciliary axoneme (By similarity).|
|Tissue Location||Most abundant in heart, brain, skeletal muscle and testis. Little expression observed in liver|
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Provided below are standard protocols that you may find useful for product applications.
Microtubule-associated proteins (MAPs) regulate microtubule stability and play critical roles in neuronal development and plasticity (1). MAP1LC3B belongs to the MAP1 LC3 family and it includes 3 different light chains, LC1, LC2 and LC3 (2). MAP1LC3B is involved in formation of autophagosomal vacuoles (autophagosomes) (3). It is most abundant in heart, brain, skeletal muscle and testis. MAP1LC3B is essential for autophagy and associated to the autophagosome membranes after processing (4).
Mandelkow E and Mandelkow EM. Microtubules and microtubule-associated proteins. Curr. Opin. Cell Biol. 1995; 7:72-81.
Fink JK, Jones SM, Esposito C, et al. Human microtubule-associated protein 1A (MAP1A) gene: genomic organization, cDNA sequence, and developmental and tissue-specific expression. Genomics 1996; 35:577-85.
Colecchia D, Strambi A, Sanzone S, et al. MAPK15/ERK8 stimulates autophagy by interacting with LC3 and GABARAP proteins. Autophagy 2012; 8:1724-40.
Kabeya Y, Mizushima N, Ueno T, et al. LC3, a mammalian homolog of yeast Apg8p, is localized in autophagosome membrane after processing. EMBO J. 2000; 19:5720-8.
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