|Application ||WB, IHC-P, IF, E|
|Other Accession||NP_060356, 151301204|
|Calculated MW||Predicted: 140 kDa |
Observed: 165 kDa
|Application Notes||ULK4 antibody can be used for detection of ULK4 by Western blot at 0.5 - 1 µg/ml. Antibody can also be used for Immunohistochemistry starting at 5 µg/mL. For immunofluorescence start at 20 µg/mL.|
|Target/Specificity||ULK4; ULK4 antibody is human and mouse reactive. Multiple isoforms of ULK4 are known to exist.|
|Reconstitution & Storage||ULK4 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year.|
|Precautions||ULK4 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||May be involved in the remodeling of cytoskeletal components, such as alpha-tubulin, and in this way regulates neurite branching and elongation, as well as cell motility.|
|Tissue Location||Expressed in the brain, mainly in postmitotic neurons, including GABAergic neurons, but not in astrocytes (at protein level).|
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Provided below are standard protocols that you may find useful for product applications.
ULK4 belongs to the Ser/Thr protein kinase superfamily and plays a role in the ATP-dependent phosphorylation of target proteins (1). Knockout of ULK genes results in a severe defect in the autophagy pathway (2). ULK4, like the other Unc-51-like kinases such as ULK1, ULK2 and ULK3, is highly conserved among eukaryotes (3). ULK4 has been implicated as a gene involved in the development of hydrocephalus (3). This neurologic disorder is being used in animal models to elucidate factors responsible for the excessive accumulation of cerebrospinal fluid in hydrocephalic humans (4).
Suzuki K, Kubota Y, Sekito T, et al. Hierarchy of Atg proteins in pre-autophagosomal structure organization. Genes to Cells 2007; 12:209–18.
Lee EJ and Tournier C. The requirement of uncoordinated 51-like kinase 1 (ULK1) and ULK2 in the regulation of autophagy. Autophagy 2011; 7:689-95.
Zhou X, Babu JR, da Silva S, et al. Unc-51-like kinase 1/2-mediated endocytic processes regulate filopodia extension and branching of sensory axons. Proc. Natl. Acad. Sci. USA 2007; 104:5842-7.
Vogel P, Read RW, Hansen GM, et al. Congenital hydrocephalus in genetically engineered mice. Vet. Pathol. 2012; 49:166-81.
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