|Application ||WB, IF, ICC, E|
|Other Accession||NP_001129502, 209862861|
|Calculated MW||Predicted: 57 kDa |
Observed: 60 kDa
|Application Notes||TESPA1 antibody can be used for detection of TESPA1 by Western blot at 1 - 2 µg/ml. Antibody can also be used for Immunocytochemistry starting at 5 µg/mL. For immunofluorescence start at 20 µg/mL.|
|Target/Specificity||TESPA1; TESPA1 antibody is human specific. At least three isoforms TESPA1 are known to exist.|
|Reconstitution & Storage||TESPA1 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year.|
|Precautions||TESPA1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Required for the development and maturation of T-cells, its function being essential for the late stages of thymocyte development (By similarity). Plays a role in T-cell antigen receptor (TCR)-mediated activation of the ERK and NFAT signaling pathways, possibly by serving as a scaffolding protein that promotes the assembly of the LAT signalosome in thymocytes. May play a role in the regulation of inositol 1,4,5-trisphosphate receptor-mediated Ca(2+) release and mitochondrial Ca(2+) uptake via the mitochondria-associated endoplasmic reticulum membrane (MAM) compartment.|
|Cellular Location||Cytoplasm. Endoplasmic reticulum membrane. Note=May localize to mitochondria- associated endoplasmic reticulum membrane (MAM)|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
email@example.com, and receive a free "I Love Antibodies" mug.
Provided below are standard protocols that you may find useful for product applications.
TESPA1 is a critical component of the TCR signalosome and is essential for T cell selection and maturation through the regulation of TCR signaling during T cell development (1). TESPA1 encodes a protein of 458 amino acids and is highly conserved among vertebrate species (2). TESPA1 expression was tightly regulated during T cell development, with highest expression at the DP stage (3). TESPA1 deficiency resulted in failure of positive selection and substantially fewer of CD4+ and CD8+ SP cells in the thymus (1,4).
Wang D, Zheng M, Lei L, et al. Tespa1 is involved in late thymocyte development through the regulation of TCR-mediated signaling. Nat. Immunol. 2012; 13:560-8.
Matsuzaki H, Fujimoto T, Ota T, et al. Tespa1 is a novel inositol 1,4,5-trisphosphate receptor binding protein in T and B lymphocytes. FEBS Open Bio. 2012; 2:255-9.
Matsuzaki H, Fujimoto T, Tanaka M, et al. Tespa1 is a novel component of mitochondria-associated endoplasmic reticulum membranes and affects mitochondrial calcium flux. Biochem. Biophys. Res. Commun. 2013; 433:322-6.
Fujimoto T, Matsuzaki H, Tanaka M, et al. Tespa1 protein is phosphorylated in response to store-operated calcium entry. Biochem. Biophys. Res. Commun. 2013; 434:162-5.
If you have used an Abgent product and would like to share how it has performed, please click on the "Submit Review" button and provide the requested information. Our staff will examine and post your review and contact you if needed.
If you have any additional inquiries please email technical services at firstname.lastname@example.org.