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TESPA1 Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, IF, ICC, E |
|---|---|
| Primary Accession | A2RU30 |
| Other Accession | NP_001129502, 209862861 |
| Reactivity | Human |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Calculated MW | Predicted: 57 kDa Observed: 60 kDa |
| Application Notes | TESPA1 antibody can be used for detection of TESPA1 by Western blot at 1 - 2 µg/ml. Antibody can also be used for Immunocytochemistry starting at 5 µg/mL. For immunofluorescence start at 20 µg/mL. |
| Gene ID | 9840 |
|---|---|
| Target/Specificity | TESPA1; TESPA1 antibody is human specific. At least three isoforms TESPA1 are known to exist. |
| Reconstitution & Storage | TESPA1 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. |
| Precautions | TESPA1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | TESPA1 |
|---|---|
| Synonyms | KIAA0748 |
| Function | Required for the development and maturation of T-cells, its function being essential for the late stages of thymocyte development (By similarity). Plays a role in T-cell antigen receptor (TCR)-mediated activation of the ERK and NFAT signaling pathways, possibly by serving as a scaffolding protein that promotes the assembly of the LAT signalosome in thymocytes. May play a role in the regulation of inositol 1,4,5-trisphosphate receptor-mediated Ca(2+) release and mitochondrial Ca(2+) uptake via the mitochondria-associated endoplasmic reticulum membrane (MAM) compartment. |
| Cellular Location | Cytoplasm. Endoplasmic reticulum membrane. Note=May localize to mitochondria- associated endoplasmic reticulum membrane (MAM) |
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Provided below are standard protocols that you may find useful for product applications.
Background
TESPA1 is a critical component of the TCR signalosome and is essential for T cell selection and maturation through the regulation of TCR signaling during T cell development (1). TESPA1 encodes a protein of 458 amino acids and is highly conserved among vertebrate species (2). TESPA1 expression was tightly regulated during T cell development, with highest expression at the DP stage (3). TESPA1 deficiency resulted in failure of positive selection and substantially fewer of CD4+ and CD8+ SP cells in the thymus (1,4).
References
Wang D, Zheng M, Lei L, et al. Tespa1 is involved in late thymocyte development through the regulation of TCR-mediated signaling. Nat. Immunol. 2012; 13:560-8.
Matsuzaki H, Fujimoto T, Ota T, et al. Tespa1 is a novel inositol 1,4,5-trisphosphate receptor binding protein in T and B lymphocytes. FEBS Open Bio. 2012; 2:255-9.
Matsuzaki H, Fujimoto T, Tanaka M, et al. Tespa1 is a novel component of mitochondria-associated endoplasmic reticulum membranes and affects mitochondrial calcium flux. Biochem. Biophys. Res. Commun. 2013; 433:322-6.
Fujimoto T, Matsuzaki H, Tanaka M, et al. Tespa1 protein is phosphorylated in response to store-operated calcium entry. Biochem. Biophys. Res. Commun. 2013; 434:162-5.
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