|Application ||WB, E|
|Other Accession||NP_065190, 40354197|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||Predicted: 35 kDa |
Observed: 38 kDa
|Application Notes||THAP11 antibody can be used for detection of THAP11 by Western blot at 1 - 2 µg/ml.|
|Target/Specificity||THAP11; THAP11 antibody is human, mouse and rat reactive.|
|Reconstitution & Storage||THAP11 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year.|
|Precautions||THAP11 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Transcriptional repressor that plays a central role for embryogenesis and the pluripotency of embryonic stem (ES) cells. Sequence-specific DNA-binding factor that represses gene expression in pluripotent ES cells by directly binding to key genetic loci and recruiting epigenetic modifiers (By similarity).|
|Cellular Location||Nucleus. Cytoplasm. Note=May be regulated by shuttling of the protein between the cytoplasm and nucleus.|
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Provided below are standard protocols that you may find useful for product applications.
The THAP domain-contining protein 11 (THAP11), also known as Ronin, is an essential factor involved in embryonic stem (ES) cell pluripotency and cell growth (1). THAP 11 contains a THAP domain, a conserved DNA-binding domain common to many proteins associated with chromatin modification and gene expression silencing, and has striking similarity to the site-specific DNA-binding domain (DBD) of Drosophila P element transposases (2). THAP11 can also negatively regulate CD44 v6 expression through its interaction with the poly(rC) binding protein PCBP1 (3).
Dejosez M, Krumenacker JS, Zitur LJ, et al. Ronin is essential for embryogenesis and the pluripotency of mouse ES cells. Cell 2008; 133:1162-74.
Roussigne M, Kossida S, Lavigne AC, et al. The THAP domain: a novel protein motif with similarity to the DNA-binding domain of P element transposase. Trends Biochem. Sci. 2003; 28:66-9.
Lian WX, Yin RH, Kong XZ, et al. THAP11, a novel binding protein of PCBP1, negatively regulates CD44 alternative splicing and cell invasion in a human hepatoma cell line. FEBS Lett. 2012; 586:1431-8.
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