|Application ||WB, IHC-P, IF, E|
|Other Accession||NP_000477, 4502179|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||Predicted: 30 kDa |
Observed: 28 kDa
|Application Notes||AQP2 antibody can be used for detection of AQP2 by Western blot at 1 - 2 µg/ml. Antibody can also be used for Immunohistochemistry starting at 5 µg/mL. For immunofluorescence start at 20 µg/mL.|
|Target/Specificity||AQP2; AQP2 antibody is human, mouse and rat reactive. This antibody is predicted to not cross-react with other members of the aquaporin protein family.|
|Reconstitution & Storage||AQP2 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year.|
|Precautions||AQP2 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Forms a water-specific channel that provides the plasma membranes of renal collecting duct with high permeability to water, thereby permitting water to move in the direction of an osmotic gradient.|
|Cellular Location||Apical cell membrane; Multi-pass membrane protein. Basolateral cell membrane; Multi-pass membrane protein Cytoplasmic vesicle membrane; Multi- pass membrane protein. Golgi apparatus, trans-Golgi network membrane; Multi-pass membrane protein. Note=Shuttles from vesicles to the apical membrane. Vasopressin-regulated phosphorylation is required for translocation to the apical cell membrane. PLEKHA8/FAPP2 is required to transport AQP2 from the TGN to sites where AQP2 is phosphorylated|
|Tissue Location||Expressed in renal collecting tubules.|
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Provided below are standard protocols that you may find useful for product applications.
Aquaporins are membrane proteins that serve in the transfer of water and small solutes across cellular membranes. One such aquaporin, aquaporin-2 (AQP2) is located in the kidney collecting tubule and plays a critical role in water reabsorbtion (1). AQP2 is mainly localized in intracellular vesicles but upon stimulation with anti-diuretic hormone (ADH), AQP2 is translocated to the apical plasma membrane by exocytic fusion of AQP2-bearing vesicles (2). Mutations in this gene have been linked to autosomal dominant and recessive forms of nephrogenic diabetes insipidus (3).
Denker BM, Smith BL, Kuhada FP, et al. Identification, purification, and partial characterization of a novel Mr 28,000 integral membrane protein from erythrocytes and renal tubules. J. Biol. Chem. 1988; 263:15634-42.
Barile M, Pisitkun T, Yu MJ, et al. Large scale protein identification in intracellular aquaporin-2 vesicles from renal inner medullary collecting duct. Mol. Cell Proteomics 2005; 4:1095-106.
Bockenhauer D and Bichet DG. Inherited secondary nephrogenic diabetes insipidus: concentrating on humans. Am. J. Physiol. Renal Physiol. 2013; 304:F1037-42.
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