|Application ||WB, E|
|Other Accession||NP_004303, 156071467|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||Predicted: 43 kDa |
Observed: 47 kDa
|Application Notes||ARR3 antibody can be used for detection of ARR3 by Western blot at 0.5 - 1 µg/ml.|
|Target/Specificity||ARR3; ARR3 antibody is human, mouse and rat reactive. At least three isoforms of ARR3 are known to exist. This antibody is predicted to not cross-react with other members of the arrestin protein family.|
|Reconstitution & Storage||ARR3 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year.|
|Precautions||ARR3 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||May play a role in an as yet undefined retina-specific signal transduction. Could binds to photoactivated-phosphorylated red/green opsins.|
|Tissue Location||Inner and outer segments, and the inner plexiform regions of the retina|
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Provided below are standard protocols that you may find useful for product applications.
Arrestin 3 (ARR3) belongs to the Arrestin family of proteins that function as negative regulators of G protein-coupled receptor (GPCR) signaling (1,2). ARR3 binds the c-Jun N-terminal kinase 3 (JNK3) and scaffolds the apoptosis signal-regulating kinase 1 (ASK1)-MAPK kinase4-JNK3 cascade in a receptor-independent fashion, promoting JNK3 phosphorylation (3,4). ARR3 also mediates the internalization of the C-C chemokine receptor CCR7 upon the binding of its ligand CCL19, but not CCL21, suggesting that it helps mediate CCR7 receptor desensitization (5).
Murakami A, Yajima T, Sakuma H, et al. X-arrestin: a new retinal arrestin mapping to the X chromosome. FEBS Lett. 1993; 334:203-9.
Gurevich VV and Gurevich EV. The structural basis of arrestin-mediated regulation of G-protein-coupled receptors. Pharmacol. Ther. 2006; 110:465-502.
Song X, Coffa S, Fu H, et al. How does arrestin assemble MAPKs into a signaling complex? J. Biol. Chem. 2009; 284:685-95.
Seo J, Tsakem EL, Breitman M, et al. Identification of arrestin-3-specific residues necessary for JNK3 kinase activation. J. Biol. Chem. 2011; 286:27894-901.
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