|Application ||WB, IHC-P, E|
|Other Accession||NP_068605, 11225256|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||Predicted: 34 kDa |
Observed: 36 kDa
|Application Notes||UCP1 antibody can be used for detection of UCP1 by Western blot at 1 - 2 µg/ml. Antibody can also be used for Immunohistochemistry starting at 2.5 µg/mL.|
|Target/Specificity||UCP1; UCP1 antibody is human, mouse and rat reactive.|
|Reconstitution & Storage||UCP1 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year.|
|Precautions||UCP1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||UCP are mitochondrial transporter proteins that create proton leaks across the inner mitochondrial membrane, thus uncoupling oxidative phosphorylation from ATP synthesis. As a result, energy is dissipated in the form of heat.|
|Cellular Location||Mitochondrion inner membrane; Multi-pass membrane protein|
|Tissue Location||Brown adipose tissue.|
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Provided below are standard protocols that you may find useful for product applications.
The mitochondrial brown fat uncoupling protein 1 (UCP1) is a member of the family of mitochondrial anion carrier proteins (MACP) (1). UCPs separate oxidative phosphorylation from ATP synthesis with energy dissipated as heat, also referred to as the mitochondrial proton leak. UCPs facilitate the transfer of anions from the inner to the outer mitochondrial membrane and the return transfer of protons from the outer to the inner mitochondrial membrane. They also reduce the mitochondrial membrane potential in mammalian cells (1). UCP1 is expressed only in brown adipose tissue, a specialized tissue which functions to produce heat (1).
1. Rial E, Gonzalez-Barroso MM, Fleury C, et al. The structure and function of the brown fat uncoupling protein UCP1: current status. Biofactors 1998; 8:209-19.
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