|Application ||WB, IHC-P, IF, E|
|Other Accession||NP_056170, 197333809|
|Calculated MW||Predicted: 81 kDa |
Observed: 96 kDa
|Application Notes||SUZ12 antibody can be used for detection of SUZ12 by Western blot at 1 - 2 µg/ml. Antibody can also be used for Immunohistochemistry starting at 5 µg/mL. For immunofluorescence start at 20 µg/mL.|
|Target/Specificity||SUZ12; SUZ12 antibody is human specific.|
|Reconstitution & Storage||SUZ12 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year.|
|Precautions||SUZ12 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Synonyms||CHET9, JJAZ1, KIAA0160|
|Function||Polycomb group (PcG) protein. Component of the PRC2/EED- EZH2 complex, which methylates 'Lys-9' (H3K9me) and 'Lys-27' (H3K27me) of histone H3, leading to transcriptional repression of the affected target gene. The PRC2/EED-EZH2 complex may also serve as a recruiting platform for DNA methyltransferases, thereby linking two epigenetic repression systems. Genes repressed by the PRC2/EED-EZH2 complex include HOXC8, HOXA9, MYT1 and CDKN2A.|
|Tissue Location||Overexpressed in breast and colon cancer.|
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Provided below are standard protocols that you may find useful for product applications.
The Polycomb group (PcG) genes contribute to the maintenance of cell identity, cell cycle regulation and oncogenesis (1). SUZ12 (suppressor of zeste 12) is a 90 - 95 kDa member of the polycomb group of transcriptional regulators (2). It is part of a transcription repression complex termed PRC2 (Polycomb repressive complex 2) that methylates histones in the nucleus, resulting in homeotic (pattern-inducing) gene silencing. It contains one C2H2-type zinc finger region and a C-terminal VEFS-box (3,4). SUZ12 is overexpressed in breast and colon cancer. SUZ12 may be a cause of endometrial stromal tumors (5).
Kirmizis A, Bartley SM, Kuzmichev A, et al. Silencing of human polycomb target genes is associated with methylation of Histone H3 Lys 27. Genes Dev. 2004; 18:1592-605.
Pasini D, Bracken AP, Hansen JB, et al. The polycomb group protein Suz12 is required for embryonic stem cell differentiation. Mol. Cell. Biol. 2007; 27:3769-79.
Cao R and Zhang Y. SUZ12 is required for both the Histone methyltransferase activity and the silencing function of the EED-EZH2 complex. Mol. Cell 2004; 15:57-67.
Pasini D, Bracken AP, Jensen MR, et al. SUZ12 is essential for mouse development and for EZH2 Histone methyltransferase activity. EMBO J. 2004; 23:4061-71.
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