|Application ||WB, IHC-P, IF, E|
|Other Accession||NP_003178, 4507351|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||Predicted: 29 kDa |
Observed: 28 kDa
|Application Notes||TAF9 antibody can be used for detection of TAF9 by Western blot at 1 - 2 µg/ml. Antibody can also be used for Immunohistochemistry at 5 µg/mL. For Immunoflorescence start at 20 µg/mL.|
|Target/Specificity||TAF9; TAF9 antibody is human, mouse and rat reactive. Multiple isoforms of TAF9 are known to exist. TAF9 is predicted to not cross-react with other TAF family members.|
|Reconstitution & Storage||TAF9 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year.|
|Precautions||TAF9 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Essential for cell viability. TAF9 and TAF9B are involved in transcriptional activation as well as repression of distinct but overlapping sets of genes. May have a role in gene regulation associated with apoptosis. TAFs are components of the transcription factor IID (TFIID) complex, the TBP-free TAFII complex (TFTC), the PCAF histone acetylase complex and the STAGA transcription coactivator-HAT complex. TFIID or TFTC are essential for the regulation of RNA polymerase II-mediated transcription.|
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Provided below are standard protocols that you may find useful for product applications.
Initiation of transcription by RNA polymerase II is coordinated by the transcription factor IID (TFIID), which binds to the core promoter to position the polymerase properly, serves as the scaffold for assembly of the remainder of the transcription complex, and acts as a channel for regulatory signals. TFIID is composed of the TATA-binding protein (TBP) and the TBP-associated factors (TAFs). TAFs may participate in basal transcription, serve as coactivators, function in promoter recognition or modify general transcription factors (GTFs) to facilitate complex assembly and transcription initiation (1). TAF9 is one of the smaller subunits of TFIID that binds to the basal transcription factor GTF2B as well as to several transcriptional activators such as p53 and VP16 (2).
Zawel L and Reinberg D. Advances in RNA polymerase II transcription. Curr. Opin. Cell Biol. 1992; 4:488-95.
Klemm RD, Goodrich JA, Zhou S, et al. Molecular cloning and expression of the 32-kDa subunit of human TFIID reveals interactions with VP16 and TFIIB that mediate transcriptional activation. Proc. Natl. Acad. Sci. USA 1995; 92:5788-92.
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