|Application ||WB, IHC-P, IF, E|
|Other Accession||NP_002387, 4505145|
|Calculated MW||Predicted: 64 kDa |
Observed: 60 kDa
|Application Notes||ME2 antibody can be used for detection of ME2 by Western blot at 1 - 2 µg/ml. Antibody can also be used for Immunohistochemistry at 5 µg/mL. For Immunoflorescence start at 20 µg/mL.|
|Target/Specificity||ME2; ME2 antibody is human specific. At least two isoforms of ME2 are known to exist; this antibody will detect both isoforms. ME2 antibody is predicted not to cross-react with ME1.|
|Reconstitution & Storage||ME2 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year.|
|Precautions||ME2 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Cellular Location||Mitochondrion matrix.|
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Provided below are standard protocols that you may find useful for product applications.
ME2 is a homotetrameric, mitochondrial NAD-dependent malic enzyme that catalyzes the oxidative decarboxylation of malate to pyruvate (1). It is related to malic enzyme 1 (ME1), a cytoplasmic NADP-dependent enzyme that generates NADPH for fatty acid biosynthesis (2). The expression of both malic enzymes is reciprocally regulated by p53; this regulation has been shown to modulate metabolism and senescence (3). Certain single-nucleotide polymorphism haplotypes of the ME2 gene have been shown to increase the risk for idiopathic generalized epilepsy (4).
Loeber G, Infante AA, Maurer-Fogy I, et al. Human NAD(+)-dependent mitochondrial malic enzyme. cDNA cloning, primary structure, and expression in Escherichia coli. J. Biol. Chem. 1991; 266:3016-21.
Gonzalez-Manchon C, Ferrer M, Ayuso MS, et al. Cloning, sequencing and functional expression of a cDNA encoding a NADP-dependent malic enzyme from human liver. Gene 1995;159:255-60.
Jiang P, Du W, Mancuso A, et al. Reciprocal regulation of p53 and malic enzymes modulates metabolism and senescence. Nature 2013; 493:689-83.
Lenzen KP, Heils A, Lorenz S, et al. Association analysis of malic acid enzyme 2 gene polymorphisms with idiopathic generalized epilepsy. Epilepsia 2005; 46:1637-41.
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