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TRIM21 Antibody

  • WB - TRIM21 Antibody ASC11777
    Western blot analysis of TRIM21 in human lung tissue lysate with TRIM21 antibody at 0.5 µg/ml in (A) the absence and (B) the presence of blocking peptide.
  • IHC - TRIM21 Antibody ASC11777
    Immunohistochemistry of TRIM21 in human small intestine tissue with TRIM21 antibody at 5 µg/mL.
  • IF - TRIM21 Antibody ASC11777
    Immunofluorescence of TRIM21 in human small intestine tissue with TRIM21 antibody at 20 µg/mL.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P19474
Other Accession NP_003132, 15208660
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype IgG
Calculated MW Predicted: 52 kDa

Observed: 52 kDa
Application Notes TRIM21 antibody can be used for detection of TRIM21 by Western blot at 0.5 - 1 µg/ml. Antibody can also be used for Immunohistochemistry at 5 µg/mL. For Immunoflorescence start at 20 µg/mL.
Additional Information
Gene ID 6737
Target/Specificity TRIM21; TRIM21 antibody is human specific. This antibody is predicted to not cross-react with TRIM6.
Reconstitution & Storage TRIM21 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year.
PrecautionsTRIM21 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name TRIM21
Synonyms RNF81, RO52, SSA1
Function E3 ubiquitin-protein ligase whose activity is dependent on E2 enzymes, UBE2D1, UBE2D2, UBE2E1 and UBE2E2. Forms a ubiquitin ligase complex in cooperation with the E2 UBE2D2 that is used not only for the ubiquitination of USP4 and IKBKB but also for its self-ubiquitination. Component of cullin-RING-based SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complexes such as SCF(SKP2)-like complexes. A TRIM21-containing SCF(SKP2)- like complex is shown to mediate ubiquitination of CDKN1B ('Thr- 187' phosphorylated-form), thereby promoting its degradation by the proteasome. Monoubiquitinates IKBKB that will negatively regulates Tax-induced NF-kappa-B signaling. Negatively regulates IFN-beta production post-pathogen recognition by polyubiquitin- mediated degradation of IRF3. Mediates the ubiquitin-mediated proteasomal degradation of IgG1 heavy chain, which is linked to the VCP-mediated ER-associated degradation (ERAD) pathway. Promotes IRF8 ubiquitination, which enhanced the ability of IRF8 to stimulate cytokine genes transcription in macrophages. Plays a role in the regulation of the cell cycle progression. Enhances the decapping activity of DCP2. Exists as a ribonucleoprotein particle present in all mammalian cells studied and composed of a single polypeptide and one of four small RNA molecules. At least two isoforms are present in nucleated and red blood cells, and tissue specific differences in RO/SSA proteins have been identified. The common feature of these proteins is their ability to bind HY RNAs.2.
Cellular Location Cytoplasm. Nucleus. Cytoplasm, P-body. Note=Enters the nucleus upon exposure to nitric oxide. Localizes to small dot- or rod-like structures in the cytoplasm, called cytoplasmic bodies (P-body) that are located underneath the plasma membrane and also diffusely in the cytoplasm and are highly motil in cells. Cytoplasmic bodies are located along the microtubules and do not share the same cytoplasmic bodies with TRIM5 Colocalizes with DCP2 in P-body
Tissue Location Isoform 1 and isoform 2 are expressed in fetal and adult heart and fetal lung
Research Areas
Citations (0)

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TRIM21, a member of the tripartite motif (TRIM) family, is a part of the RoSSA ribonucleoprotein, which includes a single polypeptide and one of four small RNA molecules. The RoSSA particle localizes to both the cytoplasm and the nucleus (1). RoSSA interacts with autoantigens in patients with Sjogren syndrome and systemic lupus erythematosus (2). TRIM21 also acts as a cytosolic antibody receptor; detection of intracellular antibodies activate immune signaling by stimulating the transcription factor pathways of NF-kappaB, AP-1, IRF3, IRF5, and IRF7, resulting in the production of proinflammatory cytokines and the induction of an antiviral state (3).


Oke V and Wahren-Herlenius M. The immunobiology of Ro52 (TRIM21) in autoimmunity: a critical review. J. Autoimmun. 2012; 39:77-82.
Frank MB, McCubbin VR, and Heldermon C. Expression and DNA binding of the human 52 kDa Ro/SSA autoantigen. Biochem. J. 1995; 305:359-62.
McEwan WA, Tam JC, Watkinson RE, et al. Intracellular antibody-bound pathogens stimulate immune signaling via the Fc receptor TRIM21. Nat. Immunol. 14:327-36.

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$ 310.00
Cat# ASC11777
(40 western blots)
Availability: 5-7days
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