|Application ||WB, IHC-P, IF, E|
|Other Accession||NP_001539, 116534937|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||Predicted: 53 kDa |
Observed: 53 kDa
|Application Notes||IFIT1 antibody can be used for detection of IFIT1 by Western blot at 1 - 2 µg/ml. Antibody can also be used for Immunohistochemistry at 5 µg/mL. For Immunoflorescence start at 20 µg/mL.|
|Target/Specificity||IFIT1; IFIT1 antibody is human, mouse and rat reactive. At least three isoforms of IFIT1 are known to exist. This antibody is predicted to not cross-react with other members of the IFIT protein family.|
|Reconstitution & Storage||IFIT1 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year.|
|Precautions||IFIT1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Synonyms||G10P1, IFI56, IFNAI1, ISG56|
|Function||Interferon-induced antiviral RNA-binding protein that specifically binds single-stranded RNA bearing a 5'-triphosphate group (PPP-RNA), thereby acting as a sensor of viral single- stranded RNAs and inhibiting expression of viral messenger RNAs. Single-stranded PPP-RNAs, which lack 2'-O-methylation of the 5' cap and bear a 5'-triphosphate group instead, are specific from viruses, providing a molecular signature to distinguish between self and non-self mRNAs by the host during viral infection. Directly binds PPP-RNA in a non-sequence-specific manner. Viruses evolved several ways to evade this restriction system such as encoding their own 2'-O-methylase for their mRNAs or by stealing host cap containing the 2'-O-methylation (cap snatching mechanism). Exhibits antiviral activity against several viruses including human papilloma and hepatitis C viruses.|
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Provided below are standard protocols that you may find useful for product applications.
The interferon-induced protein with tetratricopeptide repeats 1 (IFIT1) protein is a member of a family of tetratricopeptide repeat-containing proteins whose transcription is upregulated by interferons, virus infection, and molecular patterns such as dsRNA or lipopolysaccharides (1,2). These proteins have been suggested to induce anti-viral cellular activities in response to infection (2). Together with the interferon-induced transmembrane protein 1 (IFITM1), IFIT1 inhibits the replication of the Hepatitis C virus, suggesting that it may be useful in the development of therapeutic treatments (3).
Wathelet M, Moutschen S, Defilippi P, et al. Molecular cloning, full-length sequence and preliminary characterization of a 56-kDa protein induced by human interferons. Eur. J. Biochem. 1986; 155:11-7.
Fensterl V and Sen GC. The ISG56/IFIT1 gene family. J. Interferon Cytokine Res. 2011; 31:71-8.
Raychoudhuri A, Shrivastava S, Steele R, et al. ISG56 and IFITM1 proteins inhibit hepatitis C virus replication. J. Virol. 2011; 85:12881-9.
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