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USP25 Antibody

  • WB - USP25 Antibody ASC11789
    Western blot analysis of USP25 in mouse brain tissue lysate with USP25 antibody at 1 µg/ml in (A) the absence and (B) the presence of blocking peptide.
  • IHC - USP25 Antibody ASC11789
    Immunohistochemistry of USP25 in human brain tissue with USP25 antibody at 5 µg/mL.
  • IF - USP25 Antibody ASC11789
    Immunofluorescence of USP25 in human brain tissue with USP25 antibody at 20 µg/mL.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q9UHP3
Other Accession NP_037528, 50312666
Reactivity Human, Mouse
Host Rabbit
Clonality Polyclonal
Isotype IgG
Calculated MW Predicted: 116 kDa

Observed: 105 kDa
Application Notes USP25 antibody can be used for detection of USP25 by Western blot at 1 - 2 µg/ml. Antibody can also be used for Immunohistochemistry at 5 µg/mL. For Immunoflorescence start at 20 µg/mL.
Additional Information
Gene ID 29761
Target/Specificity USP25; USP25 antibody is human and mouse reactive. Multiple isoforms of USP25 are known to exist.
Reconstitution & Storage USP25 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year.
PrecautionsUSP25 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name USP25
Synonyms USP21
Function Deubiquitinating enzyme that hydrolyzes ubiquitin moieties conjugated to substrates and thus, functions to process newly synthesized Ubiquitin, to recycle ubiquitin molecules or to edit polyubiquitin chains and prevents proteasomal degradation of substrates. Hydrolyzes both 'Lys-48'- and 'Lys-63'-linked tetraubiquitin chains.
Cellular Location Cytoplasm
Tissue Location Isoform USB25a is found in most adult and fetal tissues; expression is moderately high in testis, pancreas, kidney, skeletal muscle, liver, lung, placenta, brain, heart, but very low in peripheral blood, colon, small intestine, ovary, prostate, thymus and spleen. Isoform USB25b is found in all tissues except heart and skeletal muscle. Isoform USB25m is heart and skeletal muscle specific.
Research Areas
Citations (0)

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USP25 (ubiquitin specific peptidase 25), also known as USP21, belongs to the peptidase C19 family and is a highly conserved 76-amino acid protein involved in regulation of intracellular protein breakdown, cell cycle regulation, and stress response (1). It contains one UBA-like domain and two UIM (ubiquitin-interacting motif) repeats. Due to alternative splicing events, USP25 is expressed as two short, ubiquitously expressed isoforms and one long, muscle-specific isoform (2). The long isoform of USP25 (USP25m) is upregulated in myogenesis and is implicated in regulation of muscular differentiation and function. USP25 is a deubiquitinating enzyme (DUB) that negatively regulates IL-17-triggered signaling (3,4).


Valero R, Marfany G, Gonzalez-Angulo O, et al. USP25, a novel gene encoding a deubiquitinating enzyme, is located in the gene-poor region 21q11.2. Genomics 1999; 62:395-405.
Valero R, Bayes M, Francisca Sanchez-Font M, et al. Characterization of alternatively spliced products and tissue-specific isoforms of USP28 and USP25. Genome Biol. 2001; 2: RESEARCH0043.
Zhong B, Liu X, Wang X, et al. Negative regulation of IL-17-mediated signaling and inflammation by the ubiquitin-specific protease USP25. Nat. Immunol. 2012; 13:1110-7.
Zhong B, Liu X, Wang X, et al. Ubiquitin-specific protease 25 regulates TLR4-dependent innate immune responses through deubiquitination of the adaptor protein TRAF3. Sci. Signal. 2013; 6:ra35.

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$ 310.00
Cat# ASC11789
(40 western blots)
Availability: 5-7days
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