DCNP1 Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| WB, IHC-P, IF, E |
---|---|
Primary Accession | Q8TF63 |
Other Accession | NP_570900, 18677770 |
Reactivity | Human, Mouse, Rat |
Host | Rabbit |
Clonality | Polyclonal |
Isotype | IgG |
Calculated MW | Predicted: 27 kDa Observed: 24 kDa |
Application Notes | DCNP1 antibody can be used for detection of DCNP1 by Western blot at 1 - 2 µg/ml. Antibody can also be used for immunohistochemistry starting at 5 µg/mL. For immunofluorescence start at 20 µg/mL. |
Gene ID | 140947 |
---|---|
Target/Specificity | C5orf20; DCNP1 antibody is human, mouse and rat reactive. |
Reconstitution & Storage | DCNP1 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. |
Precautions | DCNP1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | DCANP1 (HGNC:24459) |
---|---|
Function | Binds with and transactivates the corticotropin-releasing hormone (CRH) promoter. |
Cellular Location | Nucleus. Cytoplasm Note=Particularly on the periphery. Colocalizes with corticotropin- releasing hormone (CRH) in parvocellular neurons in the paraventricular nucleus. |
Tissue Location | Expressed in neurons of the paraventricular nucleus, thalamus and occipital cortex and in glial cells (at protein level). Predominantly expressed in dendritic cells. Detected in brain and skeletal muscle. Highly expressed in mature dendritic cells and at a lower level in immature dendritic cells. Expressed in paraventricular nucleus, supraoptic nucleus and nucleus basalis of Meynert. Strongly expressed in paraventricular nucleus of depressed patients compared to controls. Not expressed in monocytes and B-cells |
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
info@abcepta.com, and receive a free "I Love Antibodies" mug.
Provided below are standard protocols that you may find useful for product applications.
Background
The DCNP1 (dendritic cell nuclear protein 1) gene is specifically expressed in dendritic cells, potent antigen-presenting cells involved in activating naive T cells to initiate antigen-specific immune response (1). It is localized mainly in the perinucleus. One of the alleles (A/T) of this gene causes premature translation termination at aa 117 and has been associated with an increased prevalence of major depression in humans (2,3). DCNP1 may play a role in the pathogenesis of depressive disorders by enhancing corticotropin-releasing hormone expression in the hypothalamic paraventricular nucleus (4).
References
Masuda M, Senju S, Fujii Si S, et al. Identification and immunocytochemical analysis of DCNP1, a dendritic cell-associated nuclear protein. Biochem. Biophys. Res. Commun. 2002; 290:1022-9.
Willis-Owen SA, Shifman S, Copley RR, et al. DCNP1: a novel candidate gene for major depression. Mol. Psychiatry 2006; 11:121-2.
Bosker FJ, Hartman CA, Nolte IM, et al. Poor replication of candidate genes for major depressive disorder using genome-wide association data. Mol. Psychiatry 2011; 16:516-32.
Zhou T, Wang S, Ren H, et al. Dendritic cell nuclear protein-1, a novel depression-related protein, upregulates corticotropin-releasing hormone expression. Brain 2010; 133:3069-79.
If you have used an Abcepta product and would like to share how it has performed, please click on the "Submit Review" button and provide the requested information. Our staff will examine and post your review and contact you if needed.
If you have any additional inquiries please email technical services at tech@abcepta.com.