|Application ||WB, IHC-P, IF, E|
|Other Accession||NP_570900, 18677770|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||Predicted: 27 kDa |
Observed: 24 kDa
|Application Notes||DCNP1 antibody can be used for detection of DCNP1 by Western blot at 1 - 2 µg/ml. Antibody can also be used for immunohistochemistry starting at 5 µg/mL. For immunofluorescence start at 20 µg/mL.|
|Target/Specificity||C5orf20; DCNP1 antibody is human, mouse and rat reactive.|
|Reconstitution & Storage||DCNP1 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year.|
|Precautions||DCNP1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Binds with and transactivates the corticotropin- releasing hormone (CRH) promoter.|
|Cellular Location||Nucleus. Cytoplasm. Note=Particularly on the periphery Colocalizes with corticotropin-releasing hormone (CRH) in parvocellular neurons in the paraventricular nucleus|
|Tissue Location||Expressed in neurons of the paraventricular nucleus, thalamus and occipital cortex and in glial cells (at protein level). Predominantly expressed in dendritic cells Detected in brain and skeletal muscle. Highly expressed in mature dendritic cells and at a lower level in immature dendritic cells Expressed in paraventricular nucleus, supraoptic nucleus and nucleus basalis of Meynert. Strongly expressed in paraventricular nucleus of depressed patients compared to controls. Not expressed in monocytes and B-cells. EMBL; AB074498; BAB84585.1; -; mRNA EMBL; AC022092; -; NOT_ANNOTATED_CDS; Genomic_DNA EMBL; BC069608; AAH69608.1; -; mRNA EMBL; BC069610; AAH69610.1; -; mRNA EMBL; BC074831; AAH74831.1; -; mRNA EMBL; BC074832; AAH74832.1; -; mRNA CCDS; CCDS4186.1; - PIR; JC7801; JC7801 RefSeq; NP_570900.1; NM_130848.2 UniGene; Hs.152477; - ProteinModelPortal; Q8TF63; - SMR; Q8TF63; - BioGrid; 126768; 1 IntAct; Q8TF63; 2 STRING; 9606.ENSP00000421871; - iPTMnet; Q8TF63; - PhosphoSitePlus; Q8TF63; - BioMuta; DCNP1; - PaxDb; Q8TF63; - PRIDE; Q8TF63; - DNASU; 140947; - Ensembl; ENST00000503143; ENSP00000421871; ENSG00000251380 GeneID; 140947; - KEGG; hsa:140947; - UCSC; uc003lav.3; human CTD; 140947; - DisGeNET; 140947; - GeneCards; DCANP1; - HGNC; HGNC:24459; DCANP1 HPA; HPA048308; - MIM; 609710; gene neXtProt; NX_Q8TF63; - OpenTargets; ENSG00000251380; - PharmGKB; PA142672373; - eggNOG; ENOG410KHFY; Eukaryota eggNOG; ENOG4110RAG; LUCA GeneTree; ENSGT00550000075626; - HOGENOM; HOG000205565; - OMA; SHLALYQ; - OrthoDB; EOG091G0KDV; - BioCyc; ZFISH:G66-33657-MONOMER; - GenomeRNAi; 140947; - PRO; PR:Q8TF63; - Proteomes; UP000005640; Chromosome 5 Bgee; ENSG00000251380; - CleanEx; HS_C5orf20; - Genevisible; Q8TF63; HS GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW GO; GO:0006355; P:regulation of transcription, DNA-templated; IEA:UniProtKB-KW GO; GO:0006351; P:transcription, DNA-templated; IEA:UniProtKB-KW 1: Evidence at protein level; Activator; Complete proteome; Cytoplasm; DNA-binding; Nucleus; Polymorphism; Reference proteome; Transcription; Transcription regulation CHAIN 1 244 Dendritic cell nuclear protein 1 /FTId=PRO_0000229790 COMPBIAS 167 235 Ser-rich VARIANT 75 75 T -> P (in dbSNP:rs1031844) /FTId=VAR_025758 VARIANT 97 97 N -> D (in dbSNP:rs12520809) /FTId=VAR_025759 SEQUENCE 244 AA; 26704 MW; 803125CEFA61B9F8 CRC64; MHYGAATHIQ NSRSHGLETV PGHQRLERGA GGETPEFPGC HSPAPPENFG NELLPLSAPL QGLSEGLYPP GRNKTLPAGV LREGAVQFLH RGLCNSNLSS EASARPSGTQ DELHSSRRKT GQTRREGARK HLVCSFRLYP FTVHTVSPGN SHLALYQVFK AVKLCPSETS FFLSRKSLKS SDPWHPPSLS PNSWNRQAGF RAWSSHLISL SLTCSDSQSR RVSSSQQPPL HSLSSHRRAA HVPE|
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Provided below are standard protocols that you may find useful for product applications.
The DCNP1 (dendritic cell nuclear protein 1) gene is specifically expressed in dendritic cells, potent antigen-presenting cells involved in activating naive T cells to initiate antigen-specific immune response (1). It is localized mainly in the perinucleus. One of the alleles (A/T) of this gene causes premature translation termination at aa 117 and has been associated with an increased prevalence of major depression in humans (2,3). DCNP1 may play a role in the pathogenesis of depressive disorders by enhancing corticotropin-releasing hormone expression in the hypothalamic paraventricular nucleus (4).
Masuda M, Senju S, Fujii Si S, et al. Identification and immunocytochemical analysis of DCNP1, a dendritic cell-associated nuclear protein. Biochem. Biophys. Res. Commun. 2002; 290:1022-9.
Willis-Owen SA, Shifman S, Copley RR, et al. DCNP1: a novel candidate gene for major depression. Mol. Psychiatry 2006; 11:121-2.
Bosker FJ, Hartman CA, Nolte IM, et al. Poor replication of candidate genes for major depressive disorder using genome-wide association data. Mol. Psychiatry 2011; 16:516-32.
Zhou T, Wang S, Ren H, et al. Dendritic cell nuclear protein-1, a novel depression-related protein, upregulates corticotropin-releasing hormone expression. Brain 2010; 133:3069-79.
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