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MIB1 Antibody

  • WB - MIB1 Antibody ASC11794
    Western blot analysis of MIB1 in A431 cell lysate with MIB1 antibody at 1 µg/ml.
  • ICC - MIB1 Antibody ASC11794
    Immunocytochemistry of MIB1 in A431 cells with MIB1 antibody at 5 µg/mL.
  • IF - MIB1 Antibody ASC11794
    Immunofluorescence of MIB1 in A431 cells with MIB1 antibody at 20 µg/mL.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q86YT6
Other Accession NP_065825, 30348954
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Isotype IgG
Calculated MW Predicted: 89, 102, 111 kDa

Observed: 103 kDa
Application Notes MIB1 antibody can be used for detection of MIB1 by Western blot at 1 - 2 µg/ml. Antibody can also be used for Immunocytochemistry at 5 µg/mL. For Immunoflorescence start at 20 µg/mL.
Additional Information
Gene ID 57534
Target/Specificity MIB1; MIB1 antibody is human, mouse and rat reactive. At least three isoforms of MIB1 are known to exist.
Reconstitution & Storage MIB1 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year.
PrecautionsMIB1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name MIB1
Synonyms DIP1, KIAA1323, ZZANK2
Function E3 ubiquitin-protein ligase that mediates ubiquitination of Delta receptors, which act as ligands of Notch proteins. Positively regulates the Delta-mediated Notch signaling by ubiquitinating the intracellular domain of Delta, leading to endocytosis of Delta receptors. Probably mediates ubiquitination and subsequent proteasomal degradation of DAPK1, thereby antagonizing anti-apoptotic effects of DAPK1 to promote TNF- induced apoptosis (By similarity). Involved in ubiquitination of centriolar satellite CEP131, CEP290 and PCM1 proteins and hence inhibits primary cilium formation in proliferating cells. Mediates 'Lys-63'-linked polyubiquitination of TBK1, which probably participates in kinase activation.
Cellular Location Cytoplasm. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, centriolar satellite Cell membrane. Note=Localizes to the plasma membrane (By similarity). According to PubMed:15048887, it is mitochondrial, however such localization remains unclear Displaced from centriolar satellites in response to cellular stress, such as ultraviolet light (UV) radiation or heat shock
Tissue Location Widely expressed at low level. Expressed at higher level in spinal cord, ovary, whole brain, and all specific brain regions examined.
Research Areas
Citations (0)

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The E3 ubiquitin-protein ligase MIB1, also known as Mindbomb homolog 1, contains multiple ankyrin repeats and RING finger domains that functions as an E3 ubiquitin ligase (1). MIB1 interacts with and regulates the cellular expression of the death-associated protein kinase 1 (DAPK1) protein by promoting its ubiquitination and degradation (1). It also positively regulates Notch signaling by ubiquitinating the Notch receptors, thereby facilitating their endocytosis (2).


Jin Y, Blue EK, Dixon S, et al. A death-associated protein kinase (DAPK)-interacting protein, DIP-1, is an E3 ubiquitin ligase that promotes tumor necrosis factor-induced apoptosis and regulates the cellular level of DAPK. J. Biol. Chem. 2002; 277:46980-6.
Hansson EM, Lanner F, Das D, et al. Control of Notch0ligand endocytosis by ligand-receptor interaction. J. Cell Sci. 2010; 123:2931-42.

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$ 310.00
Cat# ASC11794
(40 western blots)
Availability: 5-7days
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