|Application ||WB, IHC-P, E|
|Other Accession||NP_073620, 189571664|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||Predicted: 45 kDa |
Observed: 42 kDa
|Application Notes||DEPTOR antibody can be used for detection of DEPTOR by Western blot at 1 - 2 µg/ml. Antibody can also be used for Immunohistochemistry at 5 µg/mL.|
|Target/Specificity||DEPTOR; DEPTOR antibody is human, mouse and rat reactive. At least three isoforms of DEPTOR are known to exist; this antibody will detect all three isoforms.|
|Reconstitution & Storage||DEPTOR antibody can be stored at 4℃ for three months and -20℃, stable for up to one year.|
|Precautions||DEPTOR Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Negative regulator of the mTORC1 and mTORC2 signaling pathways. Inhibits the kinase activity of both complexes.|
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Provided below are standard protocols that you may find useful for product applications.
DEPTOR is an mTOR (mammalian target of rapamycin)-interacting protein whose expression is negatively regualted by mTOR complex 1 (mTORC1) and mTORC2 (1). Loss of DEPTOR activates RP6SK1, Akt1, and SGK1, promotes cell growth and survival, and activates mTORC1 and mTORC2 kinase activities. DEPTOR expression is low in most cancers, but is often elevated in multiple myelomas (1). In respons to stimulation by mitogens, DEPTOR is rapidly phosphorylated leading to its ubiquitination and degradation, allowing mTOR to generate an auto-amplification loop to promotes its own full activation (2).
Peterson TR, Laplante M, Thoreen CC, et al. DEPTOR is an mTOR inhibitor frequently overexpressed in multiple myeloma cells and required for survival. Cell 2009; 137:873-6.
Duan S, Skaar JR, Kuchay S, et al. mTOR generates an auto-amplification loop by triggering the bTrCP- and CK1a-dependent degradation of DEPTOR. Mol. Cell 2011; 44:317-24.
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