|Application ||WB, IHC-P, IF, E|
|Other Accession||NP_115785, 65018|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||Predicted: 55 kDa |
Observed: 53 kDa
|Application Notes||PINK1 antibody can be used for detection of PINK1 by Western blot at 1 - 2 μg/ml. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.|
|Target/Specificity||PINK1 antibody was raised against a 16 amino acid peptide near the amino terminus of human PINK1.|
The immunogen is located within amino acids 120 - 170 of PINK1.
|Reconstitution & Storage||PINK1 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year.|
|Precautions||PINK1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Protects against mitochondrial dysfunction during cellular stress by phosphorylating mitochondrial proteins. Involved in the clearance of damaged mitochondria via selective autophagy (mitophagy) by mediating activation and translocation of PARK2. Targets PARK2 to dysfunctional depolarized mitochondria through the phosphorylation of MFN2. Activates PARK2 in 2 steps: (1) by mediating phosphorylation at 'Ser-65' of PARK2 and (2) mediating phosphorylation of ubiquitin, converting PARK2 to its fully-active form (PubMed:24660806, PubMed:24751536, PubMed:24784582, PubMed:25527291).|
|Cellular Location||Mitochondrion outer membrane; Single-pass membrane protein Cytoplasm, cytosol. Note=Localizes mostly in mitochondrion and the 2 proteolytic processed fragments of 55 kDa and 48 kDa localize mainly in cytosol|
|Tissue Location||Highly expressed in heart, skeletal muscle and testis, and at lower levels in brain, placenta, liver, kidney, pancreas, prostate, ovary and small intestine. Present in the embryonic testis from an early stage of development|
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Provided below are standard protocols that you may find useful for product applications.
The PTEN-induced putative kinase 1 (PINK1) is a serine/threonine protein kinase that localizes to mitochondria and is thought to protect cells from stress-induced mitochondrial dysfunction (reviewed in 1). PINK1 recruits the E3 ubiquitin ligase Parkin to mitochondria to initiate mitophagy, an autophagic process that clears damaged mitochondria within a cell (2). PINK1 is cleaved by the mitochondrial protease PARL (3). Mutations in this gene cause one form of autosomal recessive early-onset Parkinson disease (4).
Matsuda S, Kitagishi Y, and Kobayashi M. Function and characteristics of PINK1 in mitochondria. Oxid. Med. Cell. Longev. 2013;601587.
Corti O, Lesage S, and Brice A. What genetics tells us about the causes and mechanism of Parkinson’s disease. Physiol. Rev. 2011; 91:1161-218.
Deas E, Plun-Favreau H, and Gandhi S, et al. PINK1 cleavage at position A103 by the mitochondrial protease PARL. Hum. Mol. Genet. 2011; 20:867-79.
Valente EM, Abou-Sleiman PM, Caputo V, et al. Hereditary early-onset Parkinson’s disease caused by mutations in PINK1. Science 2004; 304:1158-60.
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