|Application ||WB, E|
|Other Accession||NP_002638, 34761064|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||Predicted: 91, 98 kDa |
Observed: 98 kDa
|Application Notes||PIK3C3 antibody can be used for detection of PIK3C3 by Western blot at 1 - 2 µg/ml.|
|Target/Specificity||PIK3C3; PIK3C3 antibody is human, mouse and rat reactive.|
|Reconstitution & Storage||PIK3C3 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year.|
|Precautions||PIK3C3 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Catalytic subunit of the PI3K complex that mediates formation of phosphatidylinositol 3-phosphate which plays a key role in initiation and maturation of autophagosomes. Involved in the transport of lysosomal enzyme precursors to lysosomes. Required for the abcission step in cytokinesis. Required for transport from early to late endosomes.|
|Cellular Location||Midbody. Late endosome. Note=Localizes also to discrete punctae along the ciliary axoneme and to the base of the ciliary axoneme.|
|Tissue Location||Ubiquitously expressed, with a highest expression in skeletal muscle.|
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Provided below are standard protocols that you may find useful for product applications.
PI 3-kinase p100 (phosphoinositide-3-kinase p100 subunit), also known as hVps34 or PIK3C3 (phosphoinositide-3-kinase class III), is a member of the PI3/PI4-kinase family (1). It is a catalytic subunit of the PI3K complex that mediates formation of phosphatidylinositol 3-phosphate and ubiquitously expressed with a highest expression in skeletal muscle (1,2). PIK3C3 is involved in the endosome to lysosome transport and plays important roles in intracellular membrane trafficking and autophagy (3-5).
Stopkova P, Saito T, Papolos DF, et al. Identification of PIK3C3 promoter variant associated with bipolar disorder and schizophrenia. Biol. Psychiatry 2004; 55:981-8.
Hal BS, Gabernet-Castello C, Voak A, et al. TbVps34, the trypanosome orthologue of Vps34, is required for Golgi complex segregation. J. Biol. Chem. 2006; 281:27600-12.
Backer JM. The regulation and function of class III PI3Ks: novel roles for Vps34. Biochem. J. 2008; 410:1-17.
Jaber N, Dou Z, Lin RZ, et al. Mammalian PIK3C3/VPS34: the key to autophagic processing in liver and heart. Autophagy 2012; 8:707-8.
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