|Application ||WB, IHC-P, IF, E|
|Other Accession||NP_000388, 1536|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||Predicted: 59, 63 kDa |
Observed: 58 kDa
|Application Notes||NOX2 antibody can be used for detection of NOX2 by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry starting at 2 μg/mL. For immunofluorescence start at 20 μg/mL.|
|Target/Specificity||Rabbit polyclonal NOX2 antibody was raised against a 15 amino acid peptide near the amino terminus of human NOX2.|
The immunogen is located within amino acids 140 - 190 of NOX2.
|Reconstitution & Storage||NOX2 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year.|
|Precautions||NOX2 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Critical component of the membrane-bound oxidase of phagocytes that generates superoxide. It is the terminal component of a respiratory chain that transfers single electrons from cytoplasmic NADPH across the plasma membrane to molecular oxygen on the exterior. Also functions as a voltage-gated proton channel that mediates the H(+) currents of resting phagocytes. It participates in the regulation of cellular pH and is blocked by zinc.|
|Cellular Location||Cell membrane; Multi-pass membrane protein.|
|Tissue Location||Detected in neutrophils (at protein level).|
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Provided below are standard protocols that you may find useful for product applications.
The NOX family of NAPDH oxidases is comprised of seven transmembrane proteins that oxidize intracellular NAPDH/NADH, causing electron transport across the membrane and the reduction of molecular oxygen to superoxide (1). NOX2, also known as cytochrome b beta (CYBB) is one of two proteins that make up Cytochrome b-245, thought to be a primary component of the microbicidal oxidase system of phagocytes. NOX2 deficiency is one of five described biochemical defects associated with chronic granulomatous disease (CGD) (2). Activation of the NOX2 enzyme complex in microglia is thought to be neurotoxic and may play a role in Alzheimer’s and Parkinson’s disease (3).
Bedard K and Krause KH. The Nox family of ROS-generating NAPDH oxidases: physiology and pathophysiology. Physiol. Rev. 2007; 87:245-313.
Segal AW. Cytochrome b-245 and its involvement in the molecular pathology of chronic granulomatous disease. Hematol. Oncol. North Am. 1988; 2:213-23.
Surace MJ and Block ML. Targeting microglia-mediated neurotoxicity: the potential of NOX2 inhibitors. Cell Mol. Life Sci. 2012; 69:2409-27.
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