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NOX2 Antibody

     
  • WB - NOX2 Antibody ASC11833
    Western blot analysis of PRDM16 in K562 cell lysate with PRDM16 antibody at 1 µg/mL.
  • WB - NOX2 Antibody ASC11833
    Western blot analysis of TRIF in human lung lysate with TRIF antibody at (A) 1 and (B) 2 µg/mL.
  • SPECIFICATION
  • CITATIONS
  • PROTOCOLS
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, IF, E
Primary Accession P04839
Other Accession NP_000388, 1536
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Isotype IgG
Calculated MW Predicted: 59, 63 kDa

Observed: 58 kDa
Application Notes NOX2 antibody can be used for detection of NOX2 by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry starting at 2 μg/mL. For immunofluorescence start at 20 μg/mL.
Additional Information
Gene ID 1536
Target/Specificity Rabbit polyclonal NOX2 antibody was raised against a 15 amino acid peptide near the amino terminus of human NOX2.

The immunogen is located within amino acids 140 - 190 of NOX2.
Reconstitution & Storage NOX2 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year.
PrecautionsNOX2 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name CYBB
Synonyms NOX2
Function Critical component of the membrane-bound oxidase of phagocytes that generates superoxide. It is the terminal component of a respiratory chain that transfers single electrons from cytoplasmic NADPH across the plasma membrane to molecular oxygen on the exterior. Also functions as a voltage-gated proton channel that mediates the H(+) currents of resting phagocytes. It participates in the regulation of cellular pH and is blocked by zinc.
Cellular Location Cell membrane; Multi-pass membrane protein.
Tissue Location Detected in neutrophils (at protein level).
Research Areas
Citations (0)

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Background

The NOX family of NAPDH oxidases is comprised of seven transmembrane proteins that oxidize intracellular NAPDH/NADH, causing electron transport across the membrane and the reduction of molecular oxygen to superoxide (1). NOX2, also known as cytochrome b beta (CYBB) is one of two proteins that make up Cytochrome b-245, thought to be a primary component of the microbicidal oxidase system of phagocytes. NOX2 deficiency is one of five described biochemical defects associated with chronic granulomatous disease (CGD) (2). Activation of the NOX2 enzyme complex in microglia is thought to be neurotoxic and may play a role in Alzheimer’s and Parkinson’s disease (3).

References

Bedard K and Krause KH. The Nox family of ROS-generating NAPDH oxidases: physiology and pathophysiology. Physiol. Rev. 2007; 87:245-313.
Segal AW. Cytochrome b-245 and its involvement in the molecular pathology of chronic granulomatous disease. Hematol. Oncol. North Am. 1988; 2:213-23.
Surace MJ and Block ML. Targeting microglia-mediated neurotoxicity: the potential of NOX2 inhibitors. Cell Mol. Life Sci. 2012; 69:2409-27.

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$ 320.00
Cat# ASC11833
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