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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   NOX4 Antibody   

NOX4 Antibody

  • WB - NOX4 Antibody ASC11835
    Western blot analysis of NOX4 in Jurkat cell lysate with NOX4 antibody at 1 µg/ml in (A) the absence and (B) the presence of blocking peptide.
  • IHC - NOX4 Antibody ASC11835
    Immunohistochemistry of NOX4 in human spleen tissue with NOX4 antibody at 5 µg/ml.
  • IF - NOX4 Antibody ASC11835
    Immunofluorescence of NOX4 in human spleen tissue with NOX4 antibody at 20 µg/ml.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q9NPH5
Other Accession NP_058627, 8393843
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Isotype IgG
Calculated MW Predicted: 64 kDa

Observed: 68 kDa
Application Notes NOX4 antibody can be used for detection of NOX4 by Western blot at 1 - 2 µg/ml. Antibody can also be used for Immunohistochemistry starting at 5 µg/mL. For immunofluorescence start at 20 µg/mL.
Additional Information
Gene ID 50507
Target/Specificity NOX4; NOX4 antibody is human, mouse, and rat reactive. At least four isoforms of NOX4 are known to exist. NOX4 is predicted to not cross-react with other NOX proteins.
Reconstitution & Storage NOX4 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year.
PrecautionsNOX4 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name NOX4
Synonyms RENOX
Function Constitutive NADPH oxidase which generates superoxide intracellularly upon formation of a complex with CYBA/p22phox. Regulates signaling cascades probably through phosphatases inhibition. May function as an oxygen sensor regulating the KCNK3/TASK-1 potassium channel and HIF1A activity. May regulate insulin signaling cascade. May play a role in apoptosis, bone resorption and lipolysaccharide-mediated activation of NFKB. May produce superoxide in the nucleus and play a role in regulating gene expression upon cell stimulation. Isoform 3 is not functional. Isoform 5 and isoform 6 display reduced activity.
Cellular Location Endoplasmic reticulum membrane; Multi-pass membrane protein. Cell membrane; Multi-pass membrane protein. Cell junction, focal adhesion. Note=May localize to plasma membrane and focal adhesions. According to PubMed:15927447, may also localize to the nucleus
Tissue Location Expressed by distal tubular cells in kidney cortex and in endothelial cells (at protein level). Widely expressed. Strongly expressed in kidney and to a lower extent in heart, adipocytes, hepatoma, endothelial cells, skeletal muscle, brain, several brain tumor cell lines and airway epithelial cells
Research Areas
Citations (0)

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The NOX family of NAPDH oxidases is comprised of seven transmembrane proteins that oxidize intracellular NAPDH/NADH, causing electron transport across the membrane and the reduction of molecular oxygen to superoxide (1). NOX4 is is expressed in multiple tissues and catalyzes the reduction of molecular oxygen to various reactive oxygen species (ROS) (2,3). Unlike other NOX proteins, NOX4 does not require cytosolic subunits and thus is constitutively active (4). The function of NOX4 remains unclear as it plays both protective and deleterious roles in cellular metabolism.


Bedard K and Krause KH. The Nox family of ROS-generating NAPDH oxidases: physiology and pathophysiology. Physiol. Rev. 2007; 87:245-313.
Cheng G, Cao Z, Xu X, et al. Homologs of gp91phox: cloning and tissue expression of Nox3, Nox4, and Nox5. Gene 2001; 269:131-40.
Montezano AC, Burger D, Ceravolo GS, et al. Novel Nox homologues in the vasculature: focusing on Nox4 and Nox5. Clin. Sci. 2011; 120:131-41.
Martyn KD, Frederick LM, von Loehneysen K, et al. Functional analysis of Nox4 reveals unique characteristics compared to other NAPDH oxidases. Cell. Signal. 2006; 18:69-82.

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$ 310.00
Cat# ASC11835
(40 western blots)
Availability: 5-7days
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