|Application ||WB, IHC-P, IF, E|
|Other Accession||NP_036474, 6912534|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||Predicted: 29 kDa |
Observed: 26 kDa
|Application Notes||BAMBI antibody can be used for detection of BAMBI by Western blot at 1 - 2 µg/ml. Antibody can also be used for Immunohistochemistry starting at 5 µg/mL. For immunofluorescence start at 20 µg/mL.|
|Target/Specificity||BAMBI; BAMBI antibody is human, mouse and rat reactive.|
|Reconstitution & Storage||BAMBI antibody can be stored at 4℃ for three months and -20℃, stable for up to one year.|
|Precautions||BAMBI Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Negatively regulates TGF-beta signaling.|
|Cellular Location||Membrane; Single-pass type I membrane protein|
|Tissue Location||High expression in kidney medulla, placenta and spleen; low in kidney cortex, liver, prostate and gut. Not expressed in normal skin, expression is high in melanocytes and in 3 out of 11 melanoma metastases tested|
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Provided below are standard protocols that you may find useful for product applications.
BAMBI (BMP and activin membrane-bound inhibitor homolog, NMA) is a membrane spanning glycoprotein that acts as a negative regulator of TGF-beta signaling during development (1). BAMBI transcription regulation is under the influence of beta-catenin, BMP, smad3 and smad4 (2,3). BAMBI expression can increase in colorectal and hepatocellular carcinomas relative to non-cancerous tissues (4). BAMBI is expressed at high levels during odontogenesis. BAMBI is coexpressed with Bmp-4 during early Xenopus embryogenesis and can be detected in poorly metastatic human melanoma cell lines (5).
Onichtchouk D, Chen YG, Dosch R, et al. Silencing of TGF-beta signalling by the pseudoreceptor BAMBI. Nature 1999; 401:480-5.
Yan X, Lin Z, Chen F, et al. Human BAMBI cooperates with Smad7 to inhibit transforming growth factor-beta signaling. J Biol. Chem. 2009; 284:30097-104.
Grotewold L, Plum M, Dildrop R, et al. Bambi is coexpressed with Bmp-4 during mouse embryogenesis. Mech. Dev. 2001; 100:327-30.
Fritzmann J, Morkel M, Besser D, et al. A colorectal cancer expression profile that includes transforming growth factor beta inhibitor BAMBI predicts metastatic potential. Gastroenterology 2009; 137:165-75.
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