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DAF Antibody

  • WB - DAF Antibody ASC11840
    Western blot analysis of DAF in A549 cell lysate with DAF antibody at 1 µg/ml.
  • IHC - DAF Antibody ASC11840
    Immunohistochemistry of DAF in human lung tissue with DAF antibody at 5 µg/ml.
  • IF - DAF Antibody ASC11840
    Immunofluorescence of DAF in human lung tissue with DAF antibody at 20 µg/ml.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P08174
Other Accession NP_001108224, 168693643
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype IgG
Calculated MW Predicted: 48 kDa

Observed: 49 kDa
Application Notes DAF body can be used for detection of DAF by Western blot at 1 - 2 µg/ml. Antibody can also be used for Immunohistochemistry starting at 5 µg/mL. For immunofluorescence start at 20 µg/mL.
Additional Information
Gene ID 1604
Target/Specificity CD55; DAF antibody is human specific. At least two isoforms of DAF are known to exist; this antibody will only detect the longest isoform.
Reconstitution & Storage DAF antibody can be stored at 4℃ for three months and -20℃, stable for up to one year.
PrecautionsDAF Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name CD55
Synonyms CR, DAF
Function This protein recognizes C4b and C3b fragments that condense with cell-surface hydroxyl or amino groups when nascent C4b and C3b are locally generated during C4 and c3 activation. Interaction of daf with cell-associated C4b and C3b polypeptides interferes with their ability to catalyze the conversion of C2 and factor B to enzymatically active C2a and Bb and thereby prevents the formation of C4b2a and C3bBb, the amplification convertases of the complement cascade.
Cellular Location Isoform 1: Cell membrane; Single-pass type I membrane protein Isoform 3: Secreted Isoform 5: Secreted Isoform 7: Cell membrane; Lipid-anchor, GPI-anchor
Tissue Location Expressed on the plasma membranes of all cell types that are in intimate contact with plasma complement proteins. It is also found on the surfaces of epithelial cells lining extracellular compartments, and variants of the molecule are present in body fluids and in extracellular matrix
Research Areas
Citations (0)

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The decay-accelerating factor (DAF), also known as CD55, is an integral membrane glycoprotein that is involved in the regulation of the complement cascade (1). DAF limits excessive complement activation by accelerating their decay following DAF binding, thereby disrupting the cascade and preventing damage to host cells (2). Antigens present on the DAF glycoprotein constitute the Cromer blood group system (CROM) (3).


Nicholson-Weller A, March JP, Rosen CE, et al. Surface membrane expression by human blood leukocytes and platelts of decay-accelerating factor, a regulatory protein of the complement system. Blood 1985; 65:1237-44.
Seya T and Atkinson JP. Functional properties of membrane cofactor protein of complement. Biochem. J. 1989; 64:581-8.
Storry JR, Reid ME, and Yazer MH. The Cromer blood group system: a review. Immunohematology 2010; 109-18.

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$ 310.00
Cat# ASC11840
(40 western blots)
Availability: 5-7days
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