|Application ||WB, IHC-P, IF, E|
|Other Accession||NP_055417, 23943912|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||Predicted: 149 kDa |
Observed: 140 kDa
|Application Notes||PIK3R4 antibody can be used for detection of PIK3R4 by Western blot at 1 - 2 µg/ml. Antibody can also be used for Immunohistochemistry starting at 5 µg/mL. For immunofluorescence start at 20 µg/mL.|
|Target/Specificity||PIK3R4; PIK3R4 antibody is human, mouse and rat reactive.|
|Reconstitution & Storage||PIK3R4 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year.|
|Precautions||PIK3R4 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Regulatory subunit of the PI3K complex. May regulate membrane trafficking late in the endocytic pathway.|
|Cellular Location||Late endosome. Note=Localizes also to discrete punctae along the ciliary axoneme|
|Tissue Location||Ubiquitously expressed.|
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Provided below are standard protocols that you may find useful for product applications.
PIK3R4 (phosphoinositide-3-kinase regulatory subunit 4, also known as VPS15) is a 150kD adaptor protein that enhances the lipid kinase activity of PIK3 complexes and may be involved in regulating membrane trafficking late in the endocytic pathway and autophagy (1,2). PIK3R4 is ubiquitously expressed and contains 7 WD repeats, 3 HEAT repeats and 1 protein kinase domain (1). PIK3R4 is crucial for VPS34 function and is necessary for autophagosome formation in multicellular animals and reveal an important role of VPS15 in cellular protection against ubiquitin-positive protein aggregates (3-5).
Herman PK, Stack JH, and Emr SD. A genetic and structural analysis of the yeast Vps15 protein kinase: evidence for a direct role of Vps15p in vacuolar protein delivery. EMBO J. 1991; 10:4049-60.
Stasyk OV, van der Klei IJ, Bellu AR, et al. A Pichia pastoris VPS15 homologue is required in selective peroxisome autophagy. Curr. Genet. 1999; 36:262-9.
Kihara A, Noda T, Ishihara N, et al. Two distinct Vps34 phosphatidylinositol 3-kinase complexes function in autophagy and carboxypeptidase Y sorting in Saccharomyces cerevisiae. J. Cell Biol. 2001; 152:519-30.
Stack JH, DeWald DB, Takegawa K, et al. Vesicle-mediated protein transport: regulatory interactions between the Vps15 protein kinase and the Vps34 PtdIns 3-kinase essential for protein sorting to the vacuole in yeast. J. Cell Biol. 1995; 129:321-34.
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