VEZT Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| WB, IHC-P, IF, E |
---|---|
Primary Accession | Q9HBM0 |
Other Accession | NP_060069, 155030244 |
Reactivity | Human, Mouse, Rat |
Host | Rabbit |
Clonality | Polyclonal |
Isotype | IgG |
Calculated MW | Predicted: 63, 68, 78, 81, 83, 86 kDa Observed: 60 kDa |
Application Notes | VEZT antibody can be used for detection of VEZT by Western blot at 1 - 2 µg/ml. Antibody can also be used for Immunohistochemistry starting at 5 µg/mL. For immunofluorescence start at 20 µg/mL. |
Gene ID | 55591 |
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Target/Specificity | VEZT; VEZT antibody is human, mouse and rat reactive. Multiple isoforms are known to exist. |
Reconstitution & Storage | VEZT antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. |
Precautions | VEZT Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | VEZT |
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Function | Plays a pivotal role in the establishment of adherens junctions and their maintenance in adult life. Required for morphogenesis of the preimplantation embryo, and for the implantation process. |
Cellular Location | Cell membrane; Multi-pass membrane protein. Cell projection, stereocilium membrane {ECO:0000250|UniProtKB:Q3ZK22}. Cell junction, adherens junction. Nucleus {ECO:0000250|UniProtKB:Q3ZK22}. Cytoplasmic vesicle, secretory vesicle, acrosome {ECO:0000250|UniProtKB:Q3ZK22} |
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Provided below are standard protocols that you may find useful for product applications.
Background
Vezatin (VEZT) is a transmembrane protein which localizes to adherens junctions and binds to myosin VIIA and the cadherin-catenins complex (1). Expression of this gene in gastric cancer tissues is decreased due to hypermethylation of the promoter. Expression of this gene may also be inhibited by binding of a specific microRNA to a target sequence in the 3' UTR of the transcripts (2). VEZT has also been shown to be required for morphogenesis of the preimplantation mouse embryo (3).
References
Kussel-Andermann P, El-Amraoui A, Safieddine S, et al. Vezatin, a novel transmembrane protein, bridges myosin VIIA to the cadherin-catenins complex. EMBO J. 2000; 19:6020-9.
Guo X, Jing C, Zhang L, et al. Down-regulation of VEZT gene expression in human gastric cancer involves promoter methylation and miR-43c. Biochem. Biophys. Res. Commun. 2011; 404:622-7.
Hyenne V, Louvet-Vallee S, El-Amraoui A, et al. Vezatin, a protein associated to adherins junctions, is required for mouse blastocyst morphogenesis. Dev. Biol. 2005; 287:180-91.
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