|Application ||WB, IHC-P, IF, E|
|Other Accession||NP_060069, 155030244|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||Predicted: 63, 68, 78, 81, 83, 86 kDa |
Observed: 60 kDa
|Application Notes||VEZT antibody can be used for detection of VEZT by Western blot at 1 - 2 µg/ml. Antibody can also be used for Immunohistochemistry starting at 5 µg/mL. For immunofluorescence start at 20 µg/mL.|
|Target/Specificity||VEZT; VEZT antibody is human, mouse and rat reactive. Multiple isoforms are known to exist.|
|Reconstitution & Storage||VEZT antibody can be stored at 4℃ for three months and -20℃, stable for up to one year.|
|Precautions||VEZT Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Plays a pivotal role in the establishment of adherens junctions and their maintenance in adult life. In case of Listeria infection, promotes bacterial internalization by participating in myosin VIIa recruitment to the entry site.|
|Cellular Location||Cell membrane; Multi-pass membrane protein. Cell junction, adherens junction. Nucleus. Cytoplasmic vesicle, secretory vesicle, acrosome|
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Provided below are standard protocols that you may find useful for product applications.
Vezatin (VEZT) is a transmembrane protein which localizes to adherens junctions and binds to myosin VIIA and the cadherin-catenins complex (1). Expression of this gene in gastric cancer tissues is decreased due to hypermethylation of the promoter. Expression of this gene may also be inhibited by binding of a specific microRNA to a target sequence in the 3' UTR of the transcripts (2). VEZT has also been shown to be required for morphogenesis of the preimplantation mouse embryo (3).
Kussel-Andermann P, El-Amraoui A, Safieddine S, et al. Vezatin, a novel transmembrane protein, bridges myosin VIIA to the cadherin-catenins complex. EMBO J. 2000; 19:6020-9.
Guo X, Jing C, Zhang L, et al. Down-regulation of VEZT gene expression in human gastric cancer involves promoter methylation and miR-43c. Biochem. Biophys. Res. Commun. 2011; 404:622-7.
Hyenne V, Louvet-Vallee S, El-Amraoui A, et al. Vezatin, a protein associated to adherins junctions, is required for mouse blastocyst morphogenesis. Dev. Biol. 2005; 287:180-91.
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