|Application ||WB, IHC-P, IF, E|
|Other Accession||NP_954649, 145275174|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||Predicted: 64, 70, 78 kDa |
Observed: 70 kDa
|Application Notes||KIRREL2 antibody can be used for detection of KIRREL2 by Western blot at 1 - 2 µg/ml. Antibody can also be used for immunohistochemistry starting at 5 µg/mL. For immunofluorescence start at 20 µg/mL.|
|Target/Specificity||KIRREL2; KIRREL2 antibody is human, mouse and rat reactive. At least five isoforms are are known to exist. This antibody is predicted to not cross-react with other members of the KIRREL protein family.|
|Reconstitution & Storage||KIRREL2 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year.|
|Precautions||KIRREL2 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Cellular Location||Cell membrane; Single-pass type I membrane protein|
|Tissue Location||Highly expressed in beta-cells of the pancreatic islets.|
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Provided below are standard protocols that you may find useful for product applications.
KIRREL2, also known as nephrin-like 3, is a type I transmembrane protein belonging to the immunoglobulin superfamily of cell adhesion molecules (1). KIRREL2 consists of five extracellular Ig-like repeats, a transmembrane domain, several glycosylation sites and a cytoplasmic domain that has a stretch of nine conserved residues (1,2). KIRREL2 has been found to be expressed in beta cells of pancreatic islets and is involved in the regulation of both glomerular and neural development (3,4). Downregulation of KIRREL2 is implicated in several proteinuric diseases (4,5).
Sun C, Kilburn D, Lukashin A, et al. KIRREL2, a novel immunoglobulin superfamily gene expressed primarily in beta cells of the pancreatic islets. Genomics 2003; 82:130-42.
Heikkila E, Ristola M, Havana M, et al. Trans-interaction of nephrin and Neph1/Neph3 induces cell adhesion that associates with decreased tyrosine phosphorylation of nephrin. Biochem. J. 2011; 435:619-28.
Wang H, Lehtonen S, Chen YC, et al. Neph3 associates with regulation of glomerular and neural development in zebrafish. Differentiation 2012; 83:38-46.
Mizuhara E, Minaki Y, Nakatani T, et al. Purkinje cells originate from cerebellar ventricular zone progenitors positive for Neph3 and E-cadherin. Dev. Biol. 2010; 338:202-14.
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