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Nephrin Antibody

     
  • WB - Nephrin Antibody ASC11857
    Western blot analysis of Nephrin in human kidney tissue lysate with Nephrin antibody at 1 µg/ml in (A) the absence and (B) the presence of blocking peptide.
    detail
  • IHC - Nephrin Antibody ASC11857
    Immunohistochemistry of Nephrin in human kidney tissue with Nephrin antibody at 5 µg/ml.
    detail
  • IF - Nephrin Antibody ASC11857
    Immunofluorescence of Nephrin in human kidney tissue with Nephrin antibody at 20 µg/ml.
    detail
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC, IF, E
Primary Accession O60500
Other Accession NP_004637, 4758822
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype IgG
Calculated MW Predicted: 125, 137 kDa

Observed: 125 kDa
Application Notes Nephrin antibody can be used for detection of Nephrin by Western blot at 1 - 2 µg/ml. Antibody can also be used for immunohistochemistry at 5 µg/ml. For immunofluorescence start at 20 µg/mL
Additional Information
Gene ID 4648
Target/Specificity NPHS1; Nephrin antibody is human specific.
Reconstitution & Storage Nephrin antibody can be stored at 4℃ for three months and -20℃, stable for up to one year.
PrecautionsNephrin Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name NPHS1
Synonyms NPHN
Function Seems to play a role in the development or function of the kidney glomerular filtration barrier. Regulates glomerular vascular permeability. May anchor the podocyte slit diaphragm to the actin cytoskeleton. Plays a role in skeletal muscle formation through regulation of myoblast fusion (By similarity).
Cellular Location Cell membrane; Single-pass type I membrane protein. Note=Predominantly located at podocyte slit diaphragm between podocyte foot processes. Also associated with podocyte apical plasma membrane.
Tissue Location Specifically expressed in podocytes of kidney glomeruli
Research Areas
Citations (0)
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Background

Nephrin is strongly expressed in renal glomeruli and is a member of the immunoglobulin family of cell adhesion molecules. Mutations in the Nephrin gene result in congenital nephrotic syndrome, an autosomal-recessive disorder characterized by massive proteinuria in utero and nephrosis at birth (1). Renal glomeruli allow normal kidneys to filter plasma so that it is very pure. Nephrin is expressed in the podocyte slit-diaphragm of the renal glomeruli in a manner that suggests that Nephrin molecules homodimerize in an anti-parallel fashion similar to cadherin interactions in adherens junctions. Thus, Nephrin may constitute the entire extracellular structure of the slit-diaphragm (2,3).

References

Kestila M, Lenkkeri U, Mannikko M, et al. Positionally cloned gene for a novel glomerular protein – Nephrin – is mutated in congenital nephrotic syndrome. Mol. Cell 1998; 1:575-582.
Tryggvason K. Unraveling the mechanisms of glomerular ultrafiltration: nephrin, a key component of the slit diaphragm. J. Am. Soc. Nephrol. 1999; 10:2440-5.
Tryggvason K and Wartiovaara J. Molecular basis of glomerular permselectivity. Curr. Opin. Nephrol. Hypertens. 2001; 10:543-9.

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$ 379.00
Cat# ASC11857
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