|Application ||WB, IHC-P, E|
|Other Accession||NP_001656, 46249393|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||Predicted: 21 kDa |
Observed: 23 kDa
|Application Notes||RHOG antibody can be used for detection of RHOG by Western blot at 1 - 2 µg/ml. Antibody can also be used for immunohistochemistry starting at 5 µg/mL.|
|Target/Specificity||RHOG; RHOG antibody is human, mouse and rat reactive.|
|Reconstitution & Storage||RHOG antibody can be stored at 4℃ for three months and -20℃, stable for up to one year.|
|Precautions||RHOG Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Required for the formation of membrane ruffles during macropinocytosis. Plays a role in cell migration and is required for the formation of cup-like structures during trans-endothelial migration of leukocytes. In case of Salmonella enterica infection, activated by SopB and ARHGEF26/SGEF, which induces cytoskeleton rearrangements and promotes bacterial entry.|
|Cellular Location||Cell membrane; Lipid-anchor; Cytoplasmic side|
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Provided below are standard protocols that you may find useful for product applications.
The ras homolog family member G (RHOG), also known as rho-related GTP-binding protein, is a member of the Rho family of small GTPases, which cycle between inactive GDP-bound and active GTP-bound states and function as molecular switches in signal transduction cascades (1). RHOG controls a pathway that requires the microtubule network and activates Rac1 and Cdc42Hs independently of their growth factor signaling pathway (2). RHOG is also involved in the transcriptional regulation of interferon-gamma and nuclear factor of activated T cells (NFAT) and the regulation of the actin skeleton in lymphocytes (3).
Vincent S, Janteur P, and Fort P. Growth-regulated of rhoG, a new member of the ras homolog family. Mol. Cell Biol. 1992; 12:3138-48.
Gauthier-Rouviere C, Vignal E, Meriane M, et al. RhoG GTPase controls a pathway that independently activates Rac1 and Cdc42Hs. Mol. Biol. Cell 1998; 9:1379-94.
Vigorito E, Billadeu DD, Savoy D, et al. RhoG regulates gene expression and the actin skeleton in lymphocytes. Oncogene 2003; 22:330-42.
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