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>   home   >   Products   >   Primary Antibodies   >   Cell Biology   >   WDR61 Antibody   

WDR61 Antibody

     
  • WB - WDR61 Antibody ASC11859
    Western blot analysis of WDR61 in SK-N-SH cell lysate with WDR61 antibody at 1 µg/ml.
  • IHC - WDR61 Antibody ASC11859
    Immunohistochemistry of WDR61 in mouse brain tissue with WDR61 antibody at 5 µg/ml.
  • IF - WDR61 Antibody ASC11859
    Immunofluorescence of WDR61 in mouse brain tissue with WDR61 antibody at 20 µg/ml.
  • SPECIFICATION
  • CITATIONS
  • PROTOCOLS
  • BACKGROUND
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, IF, E
Primary Accession Q9GZS3
Other Accession NP_079510, 13376840
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Isotype IgG
Calculated MW Predicted: 34 kDa

Observed: 30 kDa
Application Notes WDR61 antibody can be used for detection of WDR61 by Western blot at 1 - 2 µg/ml. Antibody can also be used for immunohistochemistry starting at 5 µg/mL. For immunofluorescence start at 20 µg/mL.
Additional Information
Gene ID 80349
Target/Specificity WDR61; WDR61 antibody is human, mouse and rat reactive.
Reconstitution & Storage WDR61 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year.
PrecautionsWDR61 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name WDR61
Function Component of the PAF1 complex (PAF1C) which has multiple functions during transcription by RNA polymerase II and is implicated in regulation of development and maintenance of embryonic stem cell pluripotency. PAF1C associates with RNA polymerase II through interaction with POLR2A CTD non- phosphorylated and 'Ser-2'- and 'Ser-5'-phosphorylated forms and is involved in transcriptional elongation, acting both indepentently and synergistically with TCEA1 and in cooperation with the DSIF complex and HTATSF1. PAF1C is required for transcription of Hox and Wnt target genes. PAF1C is involved in hematopoiesis and stimulates transcriptional activity of KMT2A/MLL1; it promotes leukemogenesis through association with KMT2A/MLL1-rearranged oncoproteins, such as KMT2A/MLL1-MLLT3/AF9 and KMT2A/MLL1-MLLT1/ENL. PAF1C is involved in histone modifications such as ubiquitination of histone H2B and methylation on histone H3 'Lys-4' (H3K4me3). PAF1C recruits the RNF20/40 E3 ubiquitin-protein ligase complex and the E2 enzyme UBE2A or UBE2B to chromatin which mediate monoubiquitination of 'Lys-120' of histone H2B (H2BK120ub1); UB2A/B-mediated H2B ubiquitination is proposed to be coupled to transcription. PAF1C is involved in mRNA 3' end formation probably through association with cleavage and poly(A) factors. In case of infection by influenza A strain H3N2, PAF1C associates with viral NS1 protein, thereby regulating gene transcription. Required for mono- and trimethylation on histone H3 'Lys-4' (H3K4me3), dimethylation on histone H3 'Lys-79' (H3K4me3). Required for Hox gene transcription. Component of the SKI complex which is thought to be involved in exosome-mediated RNA decay and associates with transcriptionally active genes in a manner dependent on PAF1C.
Cellular Location Nucleus. Cytoplasm EMBL; AF309553; AAG31639.1; -; mRNA EMBL; AF100786; AAP97225.1; -; mRNA EMBL; AF127799; AAP97249.1; -; mRNA EMBL; AK024754; BAB14986.1; -; mRNA EMBL; CR457333; CAG33614.1; -; mRNA EMBL; CH471136; EAW99174.1; -; Genomic_DNA EMBL; CH471136; EAW99175.1; -; Genomic_DNA EMBL; BC010080; AAH10080.1; -; mRNA CCDS; CCDS10300.1; - RefSeq; NP_001290176.1; NM_001303247.1 RefSeq; NP_001290177.1; NM_001303248.1 RefSeq; NP_079510.1; NM_025234.2 RefSeq; XP_011520396.1; XM_011522094.1 UniGene; Hs.513055; - PDB; 3OW8; X-ray; 2.30 A; A/B/C/D=2-305 PDBsum; 3OW8; - ProteinModelPortal; Q9GZS3; - SMR; Q9GZS3; - BioGrid; 123255; 50 CORUM; Q9GZS3; - DIP; DIP-36672N; - IntAct; Q9GZS3; 31 MINT; Q9GZS3; - STRING; 9606.ENSP00000267973; - iPTMnet; Q9GZS3; - PhosphoSitePlus; Q9GZS3; - BioMuta; WDR61; - DMDM; 74761365; - EPD; Q9GZS3; - MaxQB; Q9GZS3; - PaxDb; Q9GZS3; - PeptideAtlas; Q9GZS3; - PRIDE; Q9GZS3; - ProteomicsDB; 80126; - DNASU; 80349; - Ensembl; ENST00000267973; ENSP00000267973; ENSG00000140395 Ensembl; ENST00000558311; ENSP00000453801; ENSG00000140395 GeneID; 80349; - KEGG; hsa:80349; - UCSC; uc002bdn.4; human CTD; 80349; - EuPathDB; HostDB:ENSG00000140395.8; - GeneCards; WDR61; - HGNC; HGNC:30300; WDR61 HPA; HPA039932; - HPA; HPA040065; - MIM; 609540; gene neXtProt; NX_Q9GZS3; - OpenTargets; ENSG00000140395; - PharmGKB; PA142670595; - eggNOG; KOG4155; Eukaryota eggNOG; ENOG410XP1Z; LUCA GeneTree; ENSGT00810000125363; - HOGENOM; HOG000036765; - HOVERGEN; HBG059813; - InParanoid; Q9GZS3; - KO; K12602; - OMA; ERKCLHT; - OrthoDB; EOG091G0F6P; - PhylomeDB; Q9GZS3; - TreeFam; TF324549; - Reactome; R-HSA-112382; Formation of RNA Pol II elongation complex Reactome; R-HSA-429958; mRNA decay by 3' to 5' exoribonuclease Reactome; R-HSA-674695; RNA Polymerase II Pre-transcription Events Reactome; R-HSA-75955; RNA Polymerase II Transcription Elongation Reactome; R-HSA-8866654; E3 ubiquitin ligases ubiquitinate target proteins SignaLink; Q9GZS3; - ChiTaRS; WDR61; human EvolutionaryTrace; Q9GZS3; - GenomeRNAi; 80349; - PRO; PR:Q9GZS3; - Proteomes; UP000005640; Chromosome 15 Bgee; ENSG00000140395; - CleanEx; HS_WDR61; - ExpressionAtlas; Q9GZS3; baseline and differential Genevisible; Q9GZS3; HS GO; GO:0016593; C:Cdc73/Paf1 complex; IDA:UniProtKB GO; GO:0005737; C:cytoplasm; IDA:UniProtKB GO; GO:0005829; C:cytosol; IDA:HPA GO; GO:0005654; C:nucleoplasm; IDA:HPA GO; GO:0005634; C:nucleus; IDA:UniProtKB GO; GO:0055087; C:Ski complex; IDA:UniProtKB GO; GO:0035327; C:transcriptionally active chromatin; IDA:UniProtKB GO; GO:0043928; P:exonucleolytic nuclear-transcribed mRNA catabolic process involved in deadenylation-dependent decay; TAS:Reactome GO; GO:0080182; P:histone H3-K4 trimethylation; IMP:UniProtKB GO; GO:0045638; P:negative regulation of myeloid cell differentiation; IDA:UniProtKB GO; GO:0051571; P:positive regulation of histone H3-K4 methylation; IMP:UniProtKB GO; GO:2001162; P:positive regulation of histone H3-K79 methylation; IMP:UniProtKB GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IDA:UniProtKB GO; GO:0032968; P:positive regulation of transcription elongation from RNA polymerase II promoter; IDA:UniProtKB GO; GO:0016567; P:protein ubiquitination; TAS:Reactome GO; GO:0006366; P:transcription by RNA polymerase II; TAS:Reactome GO; GO:0006368; P:transcription elongation from RNA polymerase II promoter; TAS:Reactome GO; GO:0016055; P:Wnt signaling pathway; IEA:UniProtKB-KW Gene3D; 2.130.10.10; -; 1 InterPro; IPR020472; G-protein_beta_WD-40_rep InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf InterPro; IPR001680; WD40_repeat InterPro; IPR019775; WD40_repeat_CS InterPro; IPR017986; WD40_repeat_dom InterPro; IPR036322; WD40_repeat_dom_sf Pfam; PF00400; WD40; 6 PRINTS; PR00320; GPROTEINBRPT SMART; SM00320; WD40; 7 SUPFAM; SSF50978; SSF50978; 1 PROSITE; PS00678; WD_REPEATS_1; 1 PROSITE; PS50082; WD_REPEATS_2; 6 PROSITE; PS50294; WD_REPEATS_REGION; 1 1: Evidence at protein level; 3D-structure; Acetylation; Complete proteome; Cytoplasm; Nucleus; Reference proteome; Repeat; Transcription; Transcription regulation; WD repeat; Wnt signaling pathway CHAIN 1 305 WD repeat-containing protein 61 /FTId=PRO_0000425748 INIT_MET 1 1 Removed; alternate CHAIN 2 305 WD repeat-containing protein 61, N- terminally processed /FTId=PRO_0000245851 REPEAT 14 57 WD 1 REPEAT 62 101 WD 2 REPEAT 104 143 WD 3 REPEAT 146 187 WD 4 REPEAT 188 227 WD 5 REPEAT 230 269 WD 6 REPEAT 272 305 WD 7 MOD_RES 1 1 N-acetylmethionine MOD_RES 2 2 N-acetylthreonine; in WD repeat- containing protein 61, N-terminally processed. CONFLICT 91 91 W -> R (in Ref. 2; AAP97249) CONFLICT 176 176 D -> A (in Ref. 2; AAP97249) CONFLICT 229 229 S -> G (in Ref. 4; CAG33614) CONFLICT 255 255 S -> N (in Ref. 4; CAG33614) CONFLICT 259 259 W -> R (in Ref. 2; AAP97249) STRAND 6 11 {ECO:0000244|PDB:3OW8} STRAND 14 17 {ECO:0000244|PDB:3OW8} STRAND 19 24 {ECO:0000244|PDB:3OW8} TURN 28 30 {ECO:0000244|PDB:3OW8} STRAND 34 39 {ECO:0000244|PDB:3OW8} STRAND 44 50 {ECO:0000244|PDB:3OW8} STRAND 53 60 {ECO:0000244|PDB:3OW8} STRAND 67 72 {ECO:0000244|PDB:3OW8} STRAND 74 83 {ECO:0000244|PDB:3OW8} STRAND 86 92 {ECO:0000244|PDB:3OW8} TURN 93 96 {ECO:0000244|PDB:3OW8} STRAND 97 103 {ECO:0000244|PDB:3OW8} STRAND 112 114 {ECO:0000244|PDB:3OW8} STRAND 118 124 {ECO:0000244|PDB:3OW8} STRAND 128 134 {ECO:0000244|PDB:3OW8} TURN 135 137 {ECO:0000244|PDB:3OW8} STRAND 139 145 {ECO:0000244|PDB:3OW8} STRAND 147 149 {ECO:0000244|PDB:3OW8} STRAND 151 156 {ECO:0000244|PDB:3OW8} STRAND 160 167 {ECO:0000244|PDB:3OW8} STRAND 172 176 {ECO:0000244|PDB:3OW8} TURN 177 179 {ECO:0000244|PDB:3OW8} STRAND 182 186 {ECO:0000244|PDB:3OW8} STRAND 195 198 {ECO:0000244|PDB:3OW8} STRAND 204 208 {ECO:0000244|PDB:3OW8} STRAND 214 218 {ECO:0000244|PDB:3OW8} TURN 219 221 {ECO:0000244|PDB:3OW8} STRAND 224 228 {ECO:0000244|PDB:3OW8} STRAND 235 240 {ECO:0000244|PDB:3OW8} STRAND 244 251 {ECO:0000244|PDB:3OW8} STRAND 256 260 {ECO:0000244|PDB:3OW8} TURN 261 264 {ECO:0000244|PDB:3OW8} STRAND 265 270 {ECO:0000244|PDB:3OW8} STRAND 277 282 {ECO:0000244|PDB:3OW8} STRAND 286 293 {ECO:0000244|PDB:3OW8} STRAND 298 302 {ECO:0000244|PDB:3OW8} SEQUENCE 305 AA; 33581 MW; A26C4BBD4F8ADB80 CRC64; MTNQYGILFK QEQAHDDAIW SVAWGTNKKE NSETVVTGSL DDLVKVWKWR DERLDLQWSL EGHQLGVVSV DISHTLPIAA SSSLDAHIRL WDLENGKQIK SIDAGPVDAW TLAFSPDSQY LATGTHVGKV NIFGVESGKK EYSLDTRGKF ILSIAYSPDG KYLASGAIDG IINIFDIATG KLLHTLEGHA MPIRSLTFSP DSQLLVTASD DGYIKIYDVQ HANLAGTLSG HASWVLNVAF CPDDTHFVSS SSDKSVKVWD VGTRTCVHTF FDHQDQVWGV KYNGNGSKIV SVGDDQEIHI YDCPI
Research Areas
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Background

WDR61 (WD-repeat-containing protein 61), also known as SKI8 or REC14, is a subunit of the human PAF and SKI complexes, which function in transcriptional regulation and are involved in events downstream of RNA synthesis, such as RNA surveillance (1,2). PAF1C is implicated in regulation of development and maintenance of embryonic stem cell pluripotency. Component of the SKI complex which is thought to be involved in exosome-mediated RNA decay and associates with transcriptionally active genes in a manner dependent on PAF1C (3,4).

References

Zhu B, Mandal SS, Pham AD, et al. The human PAF complex coordinates transcription with events downstream of RNA synthesis. Genes Dev. 2005; 19:1668-73.
Zhu B, Zheng Y, Pham AD, et al. Monoubiquitination of human histone H2B: the factors involved and their roles in HOX gene regulation. Mol. Cell. 2005; 20:601-11.
Kim J, Guermah M, and Roeder RG. The human PAF1 complex acts in chromatin transcription elongation both independently and cooperatively with SII/TFIIS. Cell 2010; 140:491-503.
Chen Y, Yamaguchi Y, Tsugeno Y, et al. DSIF, the Paf1 complex, and Tat-SF1 have nonredundant, cooperative roles in RNA polymerase II elongation. Genes Dev. 2009; 23):2765-77.

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