|Application ||WB, IHC-P, IF, E|
|Other Accession||NP_001155133, 242247251|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||Predicted: 123 kDa |
Observed: 110 kDa
|Application Notes||WWC1 antibody can be used for detection of WWC1 by Western blot at 1 - 2 µg/ml. Antibody can also be used for immunohistochemistry starting at 5 µg/mL. For immunofluorescence start at 20 µg/mL.|
|Target/Specificity||WWC1; WWC1 antibody is human, mouse and rat reactive. At least three isoforms of WWC1 are known to exist; this antibody will detect all three isoforms.|
|Reconstitution & Storage||WWC1 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year.|
|Precautions||WWC1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Probable regulator of the Hippo/SWH (Sav/Wts/Hpo) signaling pathway, a signaling pathway that plays a pivotal role in tumor suppression by restricting proliferation and promoting apoptosis. Along with NF2 can synergistically induce the phosphorylation of LATS1 and LATS2 and can probably function in the regulation of the Hippo/SWH (Sav/Wts/Hpo) signaling pathway. Acts as a transcriptional coactivator of ESR1 which plays an essential role in DYNLL1-mediated ESR1 transactivation. Regulates collagen-stimulated activation of the ERK/MAPK cascade. Modulates directional migration of podocytes. Acts as a substrate for PRKCZ. Plays a role in cognition and memory performance.|
|Cellular Location||Cytoplasm. Cytoplasm, perinuclear region. Nucleus. Cell projection, ruffle membrane. Note=Colocalizes with PRKCZ in the perinuclear region|
|Tissue Location||Expressed in mammary epithelial cells and breast cancer cell lines. Found in the luminal epithelium surrounding the ducts in the normal breast. In the brain, expressed in somatodendritic compartment of neurons in the cortex and hippocampus and in the cerebellum it is found in the Purkinje cells and some granule cells (at protein level). Detected in brain, heart, colon and kidney. In the kidney, expressed in glomerular podocytes, in some tubules and in the collecting duct|
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Provided below are standard protocols that you may find useful for product applications.
The WW and C2 domain containing 1 (WWC1) protein, also known as KIBRA, possesses two WW domains and an internal C2-like domain (1). WWC1 was originally identified as a memory performance-associated protein in humans (2) and has recently been shown to be a novel regulator of the Hippo pathway (3). WWC1 is phosphorylated by the mitotic kinases Aurora-A and –B (4), and in turn activates the Aurora kinases and is required for precise chromosome alignment during mitosis (5).
Kremerskothen J, Plaas C, Buther K, et al. Characterization of KIBRA, a novel WW domain-containing protein. Biochem. Biophys. Res. Commun. 2003; 300:862-7.
Papassotiropoulos A, Stephan DA, Huentelman MJ, et al. Common Kibra alleles are associated with human memory performance. Science 2006; 314:475-8.
Genevet A, Wehr MC, Brain R, et al. Kibra is a regulator of the Salvador/Warts/Hippo signaling network. Dev. Cell 2010; 18:309-16.
Xiao L, Chen Y, Ji M, et al. KIBRA protein phosphorylation is regulated by mitotic kinase aurora and protein phosphatase 1. J. Biol. Chem. 2011; 286:36304-15.
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