|Application ||WB, IHC-P, IF, E|
|Other Accession||NP_001171656, 9737|
|Calculated MW||Predicted: 153 kDa; Observed: 150 kDa|
|Application Notes||GPRASP1 antibody can be used for detection of GPRASP1 by Western blot at 1 - 2 μg/ml. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.|
|Target/Specificity||GPRASP1 antibody was raised against a 15 amino acid peptide near the amino terminus of human GPRASP1.|
The immunogen is located within amino acids 260 - 310 of GPRASP1.
|Reconstitution & Storage||GPRASP1 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year.|
|Precautions||GPRASP1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Modulates lysosomal sorting and functional down- regulation of a variety of G-protein coupled receptors. Targets receptors for degradation in lysosomes via its interaction with BECN2.|
|Tissue Location||Expressed in the brain, with lower expression in medulla, spinal cord and substantia nigra|
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Provided below are standard protocols that you may find useful for product applications.
The G protein-coupled receptor associated sorting protein 1 (GPRASP1) is a member of the GPRASP family and is thought to modulate lysosomal sorting and functional down-regulation of a variety of G-protein coupled receptors (1,2). GPRASP1 interacts with receptors such as the D2 dopamine receptor (DRD2), delta opioid receptor (OPRD1), beta-2 adrenergic receptor (ADRB2), D4 dopamine receptor (DRD4) and cannabinoid 1 receptor (CNR1) and targets these proteins for degradation in lysosomes (2). Recently, it has been shown that increased GPRASP1 levels may act as a potential biomarker for breast cancer (3).
Simonin F, Karcher P, Boeuf JJ, et al. Identification of a novel family of G protein-coupled receptor associated proteins. J. Neurochem. 2004; 89:766-75.
Moser E, Kargl J, Whistler JL, et al. G protein-coupled receptor-associated sorting protein 1 regulates the postendocytic sorting of seven-transmembrane-spanning G protein-coupled receptors. Pharmacology 2010; 86:22-9.
Tuszynski GP, Rothman VL, Zheng X, et al. G-protein coupled receptor-associated sorting protein 1 (GASP-1), a potential biomarker in breast cancer. Exp. Mol. Pathol. 2011; 91:608-13.
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