|Application ||WB, ICC, E|
|Other Accession||NP_055684, 50582989|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||Predicted: 106 kDa |
Observed: 103 kDa
|Application Notes||IQSEC1 antibody can be used for detection of IQSEC1 by Western blot at 1 - 2 µg/ml. Antibody can also be used for immunocytochemistry at 10 µg/ml.|
|Target/Specificity||IQSEC1; IQSEC1 antibody is human, mouse and rat reactive. At least four isoforms of IQSEC1 are known to exist. IQSEC1 antibody is predicted to not cross-react with IQSEC2.|
|Reconstitution & Storage||IQSEC1 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year.|
|Precautions||IQSEC1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Synonyms||ARFGEP100, BRAG2, KIAA0763|
|Function||Guanine nucleotide exchange factor for ARF1 and ARF6 (PubMed:24058294). Guanine nucleotide exchange factor activity is enhanced by lipid binding (PubMed:24058294). Accelerates GTP binding by ARFs of all three classes. Guanine nucleotide exchange protein for ARF6, mediating internalisation of beta-1 integrin.|
|Cellular Location||Cytoplasm. Nucleus. Note=At steady state, may be preferentially cytosolic|
|Tissue Location||Expressed in brain, ovary, heart, lung, liver, kidney and leukocytes. Moderate expression was also detected in lung, skeletal muscle, placenta, small intestine, pancreas, spleen and testis.|
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Provided below are standard protocols that you may find useful for product applications.
ADP ribosylation factors (Arfs) are small GTP-binding proteins known for their role in vesicular transport, where they nucleate the assembly of coat protein complexes at sites of carrier vesicle formation. IQSEC1 (GEP100/BRAG2), a guanine nucleotide exchange protein for ARF6, belongs to the BRAG family and contains one IQ domain, one PH domain and one SEC7 domain (1). As a known activator of ARF6, IQSEC1 regulates cell surface levels of Beta1 integrin and its knockdown blocked tumour metastasis (2,3). IQSEC1 links EGFR signaling to ARF6 activation and may contribute to the cancer metastasis and malignancy (4).
Someya A, Sata M, Takeda K, et al. ARF-GEP(100), a guanine nucleotide-exchange protein for ADP-ribosylation factor 6. Proc. Natl. Acad. Sci. U.S.A. 2001; 98:2413-8.
Dunphy JL, Moravec R, Ly K, et al. The Arf6 GEF GEP100/BRAG2 regulates cell adhesion by controlling endocytosis of beta1 integrins. Curr. Biol. 2006; 16:315-20.
Moravec R, Conger KK, D'Souza R, et al. BRAG2/GEP100/IQSec1 interacts with clathrin and regulates a5B1 integrin endocytosis through activation of ADP ribosylation factor 5 (Arf5). J. Biol. Chem. 2012; 287:31138-47.
Hu Z, Du J, Yang L, et al. GEP100/Arf6 is required for epidermal growth factor-induced ERK/Rac1 signaling and cell migration in human hepatoma HepG2 cells. PLoS One 2012; 7:e38777.
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