|Application ||WB, IHC-P, E|
|Other Accession||NP_001104595, 162138911|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||Predicted: 104, 133, 141, 164 kDa |
Observed: 140 kDa
|Application Notes||IQSEC2 antibody can be used for detection of IQSEC2 by Western blot at 1 - 2 µg/ml. Antibody can also be used for immunohistochemistry starting at 5 µg/mL.|
|Target/Specificity||IQSEC2; IQSEC2 antibody is human, mouse and rat reactive. At least two isoforms of IQSEC2 are known to exist; this antibody will only detect the larger isoform. IQSEC2 antibody is predicted to not cross-react with IQSEC1.|
|Reconstitution & Storage||IQSEC2 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year.|
|Precautions||IQSEC2 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Tissue Location||Expressed in brain, kidney and small intestine. Weakly expressed in placenta, pancreas, ovary, prostate and liver.|
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Provided below are standard protocols that you may find useful for product applications.
ADP ribosylation factors (Arfs) are small GTP-binding proteins known for their role in vesicular transport, where they nucleate the assembly of coat protein complexes at sites of carrier vesicle formation. Similar to the related protein IQSEC1, IQSEC2 is a guanine nucleotide exchange protein for ARF1 and ARF6 that belongs to the BRAG family and contains one IQ domain, one PH domain and one SEC7 domain (1,2). IQSEC2 is a major component of the postsynaptic density (PSD) and colocalizes with PSD-95. It has been suggested to modulate the functions of the ARF proteins (1). Mutations in this gene have been implicated in nonsyndromic X-linked mental retardation (3).
Murphy JA, Jensen ON, and Walikonis RS. BRAG1, a SEC7 domain-containing protein, is a component of the postsynaptic density of excitatory synapses. Brain Res. 2006; 1120:35-45.
Someya A, Sata M, Takeda K, et al. ARF-GEP(100), a guanine nucleotide-exchange protein for ADP-ribosy
Shoubridge C, Tarpey PS, Abidi F, et al. Mutations in the guanine nucleotide exchange factor gene IQSEC2 cause nonsyndromic intellectual ability. Nat. Genet. 2010; 42:486-8.
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