|Application ||WB, IHC-P, IF, E|
|Other Accession||NP_001028740, 75750480|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||Predicted: 76 kDa |
Observed: 78 kDa
|Application Notes||RHOT1 antibody can be used for detection of RHOT1 by Western blot at 1 - 2 µg/ml. Antibody can also be used for immunohistochemistry starting at 5 µg/mL. For immunofluorescence start at 20 µg/mL.|
|Target/Specificity||RHOT1; RHOT1 antibody is human, mouse and rat reactive. At least three isoforms of RHOT1 are known to exist; this antibody will detect all three isoforms.|
|Reconstitution & Storage||RHOT1 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year.|
|Precautions||RHOT1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Mitochondrial GTPase involved in mitochondrial trafficking. Probably involved in control of anterograde transport of mitochondria and their subcellular distribution.|
|Cellular Location||Mitochondrion outer membrane; Single-pass type IV membrane protein. Note=Colocalizes with MGARP and RHOT2 at the mitochondria|
|Tissue Location||Ubiquitously expressed. Expressed at high level in heart and skeletal muscle.|
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Provided below are standard protocols that you may find useful for product applications.
The Ras homolog family member T1 (RHOT) is an atypical Rho Ca2+-binding GTPase that localizes to the mitochondria (1). RHOT1, the related protein RHOT2, the adaptor protein Milton, and the PTEN induced putative kinase 1 (PINK1), form a complex that is involved in axonal transport of mitochondria (2,3). Both PINK1 and Parkin target RHOT1 for phosphorylation and degradation, causing the arrest of mitochondrial motility (4).
Fransson A, Ruusala A, and Aspenstrom P. Atypical Rho GTPases have roles in mitochondrial homeostasis and apoptosis. J. Biol. Chem. 2003; 278:6495-502.
Macaskill AF, Brickley K, Stephenson FA, et al. GTPase dependent recruitment of Grif-1 by Miro1 regulates mitochondrial trafficking in hippocampal neurons. Mol. Cell. Neurosci. 2009;40:301–12.
Weihofen A, Thomas KJ, Ostaszewski B, et al. Pink1 forms a multi-protein complex with Miro and Milton, linking Pink1 function to mitochondrial trafficking. Biochemistry 2009; 48:2045-54.
Wang X, Winter D, Ashrafi G, et al. PINK1 and Parkin target Miro for phosphorylation and degradation to arrest mitochondrial motility. Cell 2011; 147:893-906.
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