|Application ||WB, IHC-P, IF, E|
|Other Accession||NP_443178, 284172479|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||Predicted: 47, 53, 56, 62 kDa |
Observed: 68 kDa
|Application Notes||NOSTRIN antibody can be used for detection of NOSTRIN by Western blot at 1 - 2 µg/ml. Antibody can also be used for immunohistochemistry starting at 5 µg/mL. For immunofluorescence start at 20 µg/mL.|
|Target/Specificity||NOSTRIN; NOSTRIN antibody is human, mouse and rat reactive. At least four isoforms are known to exist; this antibody will detect all four isoforms.|
|Reconstitution & Storage||NOSTRIN antibody can be stored at 4℃ for three months and -20℃, stable for up to one year.|
|Precautions||NOSTRIN Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Multivalent adapter protein which may decrease NOS3 activity by inducing its translocation away from the plasma membrane.|
|Cellular Location||Isoform 1: Cell membrane; Peripheral membrane protein; Cytoplasmic side Cytoplasmic vesicle. Cytoplasm, cytoskeleton. Note=Enriched in selected actin structures (PubMed:16234328, PubMed:16376344)|
|Tissue Location||Expressed at highest levels in heart, kidney, placenta and lung, and at lowest levels in brain, thymus and spleen. Present in vascular endothelial cells and placenta. Over- expressed in placenta from women with pre-eclampsia (at protein level).|
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Provided below are standard protocols that you may find useful for product applications.
NOSTRIN. also known as endothelial nitric oxide synthase traffic inducer, is a member of the Pombe Cdc15 homology (PCH) family of proteins which may function as the modulator of the subcellular localisation and activity of endothelial nitric oxide synthase (eNOS) (1,2). It decreases NOS3 activity by inducing its translocation away from the plasma membrane (3). NOSTRIN typically exists as a trimer and is expressed in the vascular endothelial cells of highly vascularized tissues such as placenta, lung, kidney and heart. NOSTRIN is necessary for proper vascular development in zebrafish and postnatal retinal angiogenesis in mice (4,5).
Zimmermann K, Opitz N, Dedio J, et al. NOSTRIN: a protein modulating nitric oxide release and subcellular distribution of endothelial nitric oxide synthase. Proc. Natl. Acad. Sci. USA 2002; 99:17167-72.
Icking A, Matt S, Opitz N, et al. NOSTRIN functions as a homotrimeric adaptor protein facilitating internalization of eNOS. J. Cell Sci. 2005; 118:5059-69.
Schilling K, Opitz N, Wiesenthal A, et al. Translocation of endothelial nitric-oxide synthase involves a ternary complex with caveolin-1 and NOSTRIN. Mol. Biol. Cell 2006; 17:3870-80.
Icking A, Schilling K, Wiesenthal A, et al. FCH/Cdc15 domain determines distinct subcellular localization of NOSTRIN. FEBS Lett. 2006; 580:223-8.
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