|Application ||WB, IHC-P, E|
|Other Accession||NP_110402, 17402919|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||Predicted: 39 kDa |
Observed: 465 kDa
|Application Notes||WNT5 antibody can be used for detection of WNT5 by Western blot at 1 - 2 µg/ml. For immunofluorescence start at 20 µg/mL.|
|Target/Specificity||WNT5; WNT5 antibody is human, mouse and rat reactive. WNT5 antibody will detect both WNT5A and WNT5B. At least two isoforms of WNT5B are known to exist; this antibody will detect both isoforms.|
|Reconstitution & Storage||WNT5 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year.|
|Precautions||WNT5 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Ligand for members of the frizzled family of seven transmembrane receptors. Probable developmental protein. May be a signaling molecule which affects the development of discrete regions of tissues. Is likely to signal over only few cell diameters (By similarity).|
|Cellular Location||Secreted, extracellular space, extracellular matrix|
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Provided below are standard protocols that you may find useful for product applications.
WNT5A and WNT5B are near-identical members of the WNT gene family, which consists of structurally related genes which encode secreted signaling proteins that have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis (1,2). WNT5A and WNT5B coordinate chondrocyte proliferation and differentiation by differentially regulating cyclin D1 and p130 expression, as well as chondrocyte-specific Col2a1 expression (3). Up-regulation of WNT5A and WNT5B has been observed in several types of human cancers, suggesting that they may be useful as diagnostic markers or potent therapeutic targets (4).
Saitoh T and Katoh M. Molecular cloning and characterization of human WNT5B on chromosome 12p13.3 region. Int. J. Oncol. 2001; 19:347-51.
Serio RN. Wnt of the two horizons: putting stem cell self-renewal and cell fate determination into context. Stem Cells Dev. 2014; epub Apr. 24.
Yang Y, Topol L, Lee H, et al. Wnt5a and Wnt5b exhibit distinct activities in coordinating chondrocyte proliferation and differentiation. Development 2003; 130:1003-15.
Saitoh T and Katoh M. Expression and regulation of WNT5A and WNT5B in human cancer: up-regulation of WNT5A by TNFalpha in MKN45 cells and up-regulation of WNT5B by beta-estradiol in MCF-7 cells. Int. J. Mol. Med. 2002; 10:345-9.
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