|Application ||WB, IHC-P, IF, E|
|Other Accession||NP_660095, 41281725|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||Predicted: 71 kDa |
Observed: 71 kDa
|Application Notes||PJA1 antibody can be used for detection of PJA1 by Western blot at 1 - 2 µg/ml. Antibody can also be used for immunohistochemistry starting at 5 µg/mL. For immunofluorescence start at 20 µg/mL.|
|Target/Specificity||PJA1; PJA1 antibody is human, mouse and rat reactive. At least two isoforms of PJA1 are known to exist; this antibody will detect both isoforms.|
|Reconstitution & Storage||PJA1 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year.|
|Precautions||PJA1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Has E2-dependent E3 ubiquitin-protein ligase activity. Ubiquitinates MAGED1 antigen leading to its subsequent degradation by proteasome (By similarity). May be involved in protein sorting.|
|Tissue Location||Expressed in various regions of the brain including the cerebellum, cerebral cortex, medulla, occipital pole, frontal lobe, temporal lobe and putamen. Highest levels in the cerebral cortex.|
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Provided below are standard protocols that you may find useful for product applications.
Ubiquitinization is an important cellular degradation process requiring sequential reactions that are mediated by three enzymes: E1, E2 and E3. PJA1, also known as Praja1 and RING finger protein 70, is a 643 amino acid E2-dependent E3-ubiquitin ligase that is abundantly expressed in the brain (1,2). Through interaction and activation with the E2-ubiquitin ligase UBC4, PJA1 mediates substrate-specific ubiquitination via its RING finger domain and facilitates ubiquitination (3). Overexpression of PJA1 in gastrointestinal cancers suggests that it may be responsible for the degradation of some anti-oncogenic proteins (4,5).
Yu P, Chen Y, Tagle DA, et al. PJA1, encoding a RING-H2 finger ubiquitin ligase, is a novel human X chromosome gene abundantly expressed in brain. Genomics 2002; 79:869-74.
Zoabi M, Sadeh R, de Bie P, et al. PRAJA1 is a ubiquitin ligase for the polycomb repressive complex 2 proteins. Biochem. Biophys. Res. Commun. 2011; 408:393-8.
Doyle JM, Gao J, Wang J, et al. MAGE-RING protein complexes comprise a family of E3 ubiquitin ligases. Mol. Cell 2010; 39:963-74.
Saha T, Vardhini D, Tang Y, et al. RING finger-dependent ubiquitination by PRAJA is dependent on TGF-beta and potentially defines the functional status of the tumor suppressor ELF. Oncogene 2006; 25:693-705.
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