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ATG4B Antibody

  • WB - ATG4B Antibody ASC11884
    Western blot analysis of ATG4B in 3T3 cell lysate with ATG4B antibody at 1 µg/ml.
  • IHC - ATG4B Antibody ASC11884
    Immunohistochemistry of ATG4B in human spleen tissue with ATG4B antibody at 5 µg/ml.
  • IF - ATG4B Antibody ASC11884
    Immunofluorescence of ATG4B in human spleen tissue with ATG4B antibody at 20 µg/ml.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q9Y4P1
Other Accession NP_037457, 47132611
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Isotype IgG
Calculated MW Predicted: 43 kDa

Observed: 44 kDa
Application Notes ATG4B antibody can be used for detection of ATG4B by Western blot at 1 - 2 µg/ml. Antibody can also be used for immunohistochemistry starting at 5 µg/mL. For immunofluorescence start at 20 µg/mL.
Additional Information
Gene ID 23192
Target/Specificity ATG4B; ATG4B antibody is human, mouse and rat reactive. At least two isoforms of ATG4B are known to exist; this antibody will detect only the larger isoform. ATG4B is predicted to not cross-react with other ATG4 proteins.
Reconstitution & Storage ATG4B antibody can be stored at 4℃ for three months and -20℃, stable for up to one year.
PrecautionsATG4B Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name ATG4B
Synonyms APG4B, AUTL1, KIAA0943
Function Cysteine protease required for the cytoplasm to vacuole transport (Cvt) and autophagy. Cleaves the C-terminal amino acid of ATG8 family proteins MAP1LC3, GABARAPL1, GABARAPL2 and GABARAP, to reveal a C-terminal glycine. Exposure of the glycine at the C- terminus is essential for ATG8 proteins conjugation to phosphatidylethanolamine (PE) and insertion to membranes, which is necessary for autophagy. Has also an activity of delipidating enzyme for the PE-conjugated forms.
Cellular Location Cytoplasm.
Tissue Location Mainly expressed in the skeletal muscle, followed by brain, heart, liver and pancreas
Research Areas
Citations (0)

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Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components (1,2). ATG4B, also known as AUTL1, is one of four mammalian orthologs of the yeast ATG4 protein; all four are cysteine proteases (3). ATG4 is required for ATG8 conjugation to phosphatidylethanolamine on autophagosomal membranes. In mammals, each ATG4 homolog shows a selective preference for the ATG8 homologs (4). ATG4B has been found to be a novel protective protein in inflammatory colitis (5).


Gozuacik D and Kimchi A. Autophagy as a cell death and tumor suppressor mechanism. Oncogene 2004; 23:2891-906.
Kisen GO, Tessitore L, Costelli P, et al. Reduced autophagic activity in primary rat hepatocellular carcinoma and ascites hepatoma cells. Carcinogenesis 1993; 14:2501-5.
Marino G, Uria JA, Puente XS, et al. Human autophagins, a family of cysteine proteinases potentially implicated in cell degradation by autophagy. J. Biol. Chem. 2003; 278:3671-8.
Li M, Hou Y, Wang J, et al. Kinetic comparisons of mammalian Atg4 homologues indicate selective preferences towards diverse Atg8 substrates. J. Biol. Chem. 2011; 286:7327-38.

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$ 310.00
Cat# ASC11884
(40 western blots)
Availability: 5-7days
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