|Application ||WB, IHC-P, E|
|Other Accession||NP_115964, 110431364|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||Predicted: 58 kDa |
Observed: 60 kDa
|Application Notes||GLIS2 antibody can be used for detection of GLIS2 by Western blot at 1 - 2 µg/ml. Antibody can also be used for immunohistochemistry starting at 5 µg/mL.|
|Target/Specificity||GLIS2; GLIS2 antibody is human, mouse and rat reactive.|
|Reconstitution & Storage||GLIS2 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year.|
|Precautions||GLIS2 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Can act either as a transcriptional repressor or as a transcriptional activator, depending on the cell context. Acts as a repressor of the Hedgehog signaling pathway (By similarity). Represses the Hedgehog-dependent expression of Wnt4 (By similarity). Necessary to maintain the differentiated epithelial phenotype in renal cells through the inhibition of SNAI1, which itself induces the epithelial-to-mesenchymal transition (By similarity). Represses transcriptional activation mediated by CTNNB1 in the Wnt signaling pathway. May act by recruiting the corepressors CTBP1 and HDAC3. May be involved in neuron differentiation (By similarity).|
|Cellular Location||Nucleus speckle. Cytoplasm|
|Tissue Location||Expressed at high levels in kidney and at low levels in heart, lung and placenta. Expressed in colon|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
firstname.lastname@example.org, and receive a free "I Love Antibodies" mug.
Provided below are standard protocols that you may find useful for product applications.
GLIS2, also known as neuronal Krueppel-like, is a 524 amino acid protein that belongs to the GLI C2H2-type zinc-finger protein family (1). GLIS2 can act either as a transcription repressor or as a transcription activator and may be involved in neuron differentiation (1,2). GLIS2 is expressed at high levels in kidney and at low levels in heart, lung and placenta. Mutations of GLIS2 have been suggested to be associated with development of progressive chronic kidney disease with characteristics resembling nephronophthisis (3,4).
Vasanth S, ZeRuth G, Kang HS, et al. Identification of nuclear localization, DNA binding, and transactivating mechanisms of Kruppel-like zinc finger protein Gli-similar 2 (Glis2). J. Biol. Chem. 2011; 286:4749-59.
Hosking CR, Ulloa F, Hogan C, et al. The transcriptional repressor Glis2 is a novel binding partner for p120 catenin. Mol. Biol. Cell 2007; 18:1918-27.
Kim YS, Kang HS, Herbert R, et al. Kruppel-like zinc finger protein Glis2 is essential for the maintenance of normal renal functions. Mol. Cell Biol. 2008; 28:2358-67.
Attanasio M, Uhlenhaut NH, Sousa VH, et al. Loss of GLIS2 causes nephronophthisis in humans and mice by increased apoptosis and fibrosis. Nat. Genet. 2007; 39:1018-24.
If you have used an Abgent product and would like to share how it has performed, please click on the "Submit Review" button and provide the requested information. Our staff will examine and post your review and contact you if needed.
If you have any additional inquiries please email technical services at email@example.com.