|Application ||WB, IHC-P, E|
|Other Accession||NP_056083, 112421122|
|Calculated MW||Predicted: 249 kDa |
Observed: 249 kDa
|Application Notes||DNAJC13 antibody can be used for detection of DNAJC13 by Western blot at 1 - 2 µg/mL. Antibody can also be used for immunohistochemistry starting at 5 µg/mL.|
|Target/Specificity||DNAJC13; DNAJC13 antibody is human and mouse reactive. At least two siforms of DNAJC13 are known to exist; this antibody will detect both isoforms. DNAJC13 antibody is predicted to not cross-react with other DNAJC family members.|
|Reconstitution & Storage||DNAJC13 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year.|
|Precautions||DNAJC13 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Involved in membrane trafficking through early endosomes, such as the early endosome to recycling endosome transport implicated in the recycling of transferrin and the early endosome to late endosome transport implicated in degradation of EGF and EGFR (PubMed:18256511, PubMed:18307993). Involved in the regulation of endosomal membrane tubulation and regulates th dynamics of SNX1 on the endosomal membrane; via association with FAM21 may link the WASH complex to the retromer SNX-BAR subcomplex (PubMed:24643499).|
|Cellular Location||Early endosome. Early endosome membrane; Peripheral membrane protein. Endosome membrane|
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Provided below are standard protocols that you may find useful for product applications.
DNAJC13, also known as receptor-mediated endocytosis 8 (RME8), is the human homolog to a DnaJ domain-containing protein originally identified in a screen for endocytic defects in C. elegans (1). It is thought to be a co-chaperone of Hsc70 which regulates protein conformation at membrane sites and plays a role in intracellular trafficking, co-localizing with markers of the endosomal system. Recent experiments have indicated that the DNAJC13 protein is involved in membrane trafficking through early endosomes but not through degradative organelles (2). DNAJC13 has been also been shown to regulate the intracellular trafficking of the epidermal growth factor receptor (3).
Girard M, Poupon V, Blondeau F, et al. The DnaJ-domain protein RME-8 functions in endosomal trafficking. J. Biol. Chem. 2005; 280:40135-43.
Fujibayashi A, Taguchi T, Misaki R, et al. Human RME-8 is involved in membrane trafficking through early endosomes. Cell Struct. Funct. 2008; 33:35-50.
Girard M and McPherson PS. RME-8 regulates trafficking of the epidermal growth factor receptor. FEBS Lett. 2008; 582:961-6.
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