|Application ||WB, IHC-P, IF, E|
|Other Accession||NP_000667, 4501951|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||Predicted: 37 kDa |
Observed: 41 kDa
|Application Notes||ADORA2B antibody can be used for detection of ADORA2B by Western blot at 0.5 - 1 µg/mL. Antibody can also be used for immunohistochemistry starting at 5 µg/mL. For immunofluorescence start at 20 µg/mL.|
|Target/Specificity||ADORA2B; ADORA2B antibody is human, mouse and rat reactive. At least two isoforms of ADORA2B are known to exist; this antibody will detect both isoforms. ADORA2B antibody is predicted to not cross-react with ADORA2A.|
|Reconstitution & Storage||ADORA2B antibody can be stored at 4℃ for three months and -20℃, stable for up to one year.|
|Precautions||ADORA2B Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Receptor for adenosine. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase.|
|Cellular Location||Cell membrane; Multi-pass membrane protein.|
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Provided below are standard protocols that you may find useful for product applications.
The adenosine receptor ADORA2B is a member of the G protein-coupled receptor superfamily and is an integral membrane protein that stimulates adenylate cyclase activity in the presence of adenosine (1,2). Extracellular adenosine triggers a potent anti-inflammatory response that is mediated in part by ADORA2B including the stimulation of IL-10 production (3-5). Activation of ADORA2B can also enhance the abundance of regulatory T cells (Tregs), a class of cells that work to constrain inflammation (6).
Pierce KD, Furlong TJ, Selbie LA, et al. Molecular cloning and expression of an adenosine A2b receptor from human brain. Biochem. Biophys. Res. Commun. 1992; 187:86-93.
Thimm D, Schiedel AC, Sherbiny FF, et al. Ligand-specific binding and activation of the human adenosine A(2B) receptor. Biochem. 2013; 52:726-40.
Blackburn MR, Vance CO, Morschl E, et al. Adenosine receptors and inflammation. Handb. Exp. Pharmacol. 2009; 215-69.
Grenz A, Homann D, Eltzschig HK. Extracellular adenosine: a safety signal that dampens hypoxia-induced inflammation during ischemia. Antioxid. Redox Signal. 2011; 15:2221-34.
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