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CRBN Antibody

     
  • WB - CRBN Antibody ASC11899
    Western blot analysis of CRBN in human testis tissue lysate with CRBN antibody at (A) 0.5 and (B) 1 µg/ml.
  • IHC - CRBN Antibody ASC11899
    Immunohistochemistry of CRBN in rat testis tissue with CRBN antibody at 5 µg/mL.
  • IF - CRBN Antibody ASC11899
    Immunofluorescence of CRBN in rat testis tissue with CRBN antibody at 20 µg/mL.
  • SPECIFICATION
  • CITATIONS
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  • BACKGROUND
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, IF, E
Primary Accession Q96SW2
Other Accession NP_057386, 39545580
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Isotype IgG
Calculated MW Predicted: 49 kDa

Observed: 54 kDa
Application Notes CRBN antibody can be used for detection of CRBN by Western blot at 1 - 2 µg/mL. Antibody can also be used for immunohistochemistry starting at 5 µg/mL. For immunofluorescence start at 20 µg/mL.
Additional Information
Gene ID 51185
Target/Specificity CRBN; CRBN antibody is human, mouse and rat reactive. At least two isoforms of CRBN are known to exist; this antibody will detect both isoforms.
Reconstitution & Storage CRBN antibody can be stored at 4℃ for three months and -20℃, stable for up to one year.
PrecautionsCRBN Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name CRBN
Function Substrate recognition component of a DCX (DDB1-CUL4-X- box) E3 protein ligase complex that mediates the ubiquitination and subsequent proteasomal degradation of target proteins, such as MEIS2. Normal degradation of key regulatory proteins is required for normal limb outgrowth and expression of the fibroblast growth factor FGF8. May play a role in memory and learning by regulating the assembly and neuronal surface expression of large-conductance calcium-activated potassium channels in brain regions involved in memory and learning via its interaction with KCNT1. Binding of pomalidomide and other thalidomide-related drugs changes the substrate specificity of the human protein, leading to decreased degradation of MEIS2 and other target proteins and increased degradation of MYC, IRF4, IKZF1 and IKZF3.
Cellular Location Cytoplasm. Nucleus. Membrane; Peripheral membrane protein
Tissue Location Widely expressed. Highly expressed in brain.
Research Areas
Citations (0)

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Background

CRBN, a member of the Lon protease protein family, plays a role in brain development (1). It is a component of the DCX (DDB1-CUL4-X-box) E3 protein ligase complex, a complex that mediates the ubiquitination and subsequent proteasomal degradation of target proteins and is required for limb outgrowth and expression of the fibroblast growth factor FGF8 (2). CRBN is thought to regulate the assembly and neuronal surface expression of large-conductance calcium-activated potassium channels in brain regions involved in memory and learning via its interaction with KCNT1. It is widely expressed and highly expressed in brain (3,4).

References

Xin W, Xiaohua N, Peilin C, et al. Primary function analysis of human mental retardation related gene CRBN. Mol. Biol. Rep. 2008; 35:251-6.
Gandhi AK, Kang J, Havens CG, et al. Immunomodulatory agents lenalidomide and pomalidomide co-stimulate T cells by inducing degradation of T cell repressors Ikaros and Aiolos via modulation of the E3 ubiquitin ligase complex CRL4(CRBN). Br. J. Haematol. 2014; 164:811-21.
Liu J, Ye J, Zou X, et al. CRL4A (CRBN) E3 ubiquitin ligase restricts BK channel activity and prevents epileptogenesis. Nat. Commun. 2014; 5:3924.
Heintel D, Rocci A, Ludwig H, et al. High expression of cereblon (CRBN) is associated with improved clinical response in patients with multiple myeloma treated with lenalidomide and dexamethasone. Br. J. Haematol. 2013; 161:695-700.

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$ 320.00
Cat# ASC11899
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